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目的探讨四君子汤常态制剂及纳米制剂对急性肝功能衰竭小鼠肠屏障功能的影响。方法 BALB/c小鼠经腹腔一次性注射D-GaIN/LPS,制备急性肝衰竭致肠屏障损伤模型,观察四君子汤常态制剂及其纳米制剂对肠道正常菌群,肠黏膜分泌型免疫球蛋白A(sIgA)含量,肠组织二胺氧化酶(DAO)活性及肝功能的影响。结果小鼠腹腔注射D-GaIN/LPS后,血清中ALT、AST活性显著提高(P<0.01);肠道内肠杆菌、肠球菌数量明显上升(P<0.01),乳酸杆菌、双歧杆菌数量明显下降(P<0.05);肠黏膜sIgA及肠组织DAO含量明显降低(P<0.01)。治疗组上述指标均得到明显改善,与自然恢复组比较差异有统计学意义(P<0.05,P<0.01);治疗组间比较四君子汤纳米制剂效果要好于常态制剂。结论急性肝功能衰竭时肠屏障明显受损,四君子汤常态制剂和纳米制剂均具有一定的保护作用,但纳米制剂效果优于常态制剂,且用药量小。
Objective To investigate the effect of Sijunzi Decoction and its nano-preparation on intestinal barrier function in mice with acute hepatic failure. Methods BALB / c mice were injected intraperitoneally with D-GaIN / LPS to establish the model of intestinal barrier injury induced by acute hepatic failure. The normal intestinal flora and its nanostructures were used to observe the effects of Sijunzi Decoction and its nano-preparation on normal intestinal flora, secretory immunoglobulin A (sIgA) content, intestinal tissue diamine oxidase (DAO) activity and liver function. Results After intraperitoneal injection of D-GaIN / LPS, the activity of ALT and AST in serum increased significantly (P <0.01). The numbers of enterobacteria and enterococci in the intestine increased significantly (P <0.01), the number of Lactobacillus and Bifidobacterium significantly increased Decreased (P <0.05). The intestinal mucosa sIgA and intestinal DAO content decreased significantly (P <0.01). The above indicators in the treatment group were significantly improved, compared with the natural recovery group, the difference was statistically significant (P <0.05, P <0.01); compared with the Sijunzi decoction of the treatment group was better than the normal preparation. Conclusion The intestinal barrier is obviously damaged in acute liver failure. Sijunzi Decoction and nano-preparation have some protective effects. However, the effect of nano-preparation is better than that of normal preparation and the dosage is small.