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目的:探讨乳腺癌患者体内一群髓系来源抑制性细胞(MDSCs)的表型特点及它对淋巴细胞的抑制作用。方法:收集20例乳腺癌患者外周血及肿瘤组织,10例体检正常健康者外周血,通过流式和免疫组织化学染色技术检测MDSCs的表型特点及比例。采用免疫磁珠技术分选乳腺癌患者肿瘤中的MDSCs,通过瑞氏染色观察细胞形态,分别利用MTT法、流式细胞术和ELISA法检测其对自体淋巴细胞增殖、凋亡和细胞因子分泌的影响。结果:流式细胞仪检测结果显示乳腺癌患者肿瘤组织及外周血中有一群具有髓系前体细胞形态的MDSCs,表面标志为CD45+CD13+CD33+CD14-CD15-。实验结果显示肿瘤组织来源的MDSCs显著抑制自体淋巴细胞增殖,促进其调亡,并显著抑制其分泌IFN-γ,促进TGF-β、IL-10释放。结论:乳腺癌患者肿瘤组织中存在一群具有独特表型特点的MDSCs,不仅抑制自体淋巴细胞增殖和因子分泌,同时促进细胞凋亡。
Objective: To investigate the phenotypic characteristics of a group of myeloid derived inhibitory cells (MDSCs) in breast cancer patients and its inhibitory effect on lymphocytes. Methods: Peripheral blood and tumor tissue were collected from 20 patients with breast cancer and 10 healthy volunteers were examined by flow cytometry and immunohistochemical staining. Immunomagnetic beads were used to sort the MDSCs in breast cancer patients. The morphology of the cells was observed by Wright staining. The proliferation, apoptosis and cytokine secretion of autologous lymphocytes were detected by MTT, flow cytometry and ELISA, respectively influences. Results: The results of flow cytometry showed that there was a group of MDSCs with myeloid progenitor cells in the tumor tissue and peripheral blood of breast cancer patients. The surface markers were CD45 + CD13 + CD33 + CD14-CD15-. The experimental results showed that MDSCs derived from tumor tissue significantly inhibited autologous lymphocyte proliferation, promoted apoptosis, significantly inhibited the secretion of IFN-γ, and promoted the release of TGF-β and IL-10. Conclusion: There are a group of MDSCs with unique phenotype in breast cancer patients, which not only inhibit autologous lymphocyte proliferation and factor secretion, but also promote apoptosis.