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目的 研究芦荟素、肉桂酸、苦参碱等 15种单体化学物对酪氨酸酶活性的影响 ,判定其抑制类型 ,从而为色素增加性皮肤病和外用皮肤脱色剂提供药物。方法 选用酪氨酸酶多巴速率氧化法体外测定药物干预前后酪氨酸酶活性 ,计算酪氨酸酶抑制率 ,分别作出Lineweaver Burk曲线 ,推断其抑制类型。结果 15种中药单体化学物中 ,芦荟素、肉桂酸和苦参碱能显著抑制酪氨酸酶活性 ,其中芦荟素 (2mmol/L)、苦参碱 (2mmol/L和 0 5mmol/L)酪氨酸酶的抑制率高于氢醌 (0 5mmol/L) (P <0 0 5 )。结论 芦荟素、肉桂酸、苦参碱均可下调酪氨酸酶活性。苦参碱属于酪氨酸酶非竞争性抑制 ,芦荟素、肉桂酸属于混合型抑制剂。
Objective To study the effects of 15 monomer chemicals such as aloin, cinnamic acid and matrine on tyrosinase activity and determine the type of inhibition, thus providing drugs for pigmented dermatosis and topical skin depigmentation agents. Methods The tyrosinase activity of tyrosinase was measured by using tyrosinase Dopa rate oxidation method in vitro and the tyrosinase inhibition rate was calculated. Lineweaver Burk curve was made and the type of inhibition was inferred. Results The results showed that aloin, cinnamic acid and matrine could significantly inhibit tyrosinase activity in 15 kinds of Chinese herbal monomer chemicals, including aloin (2mmol/L) and matrine (2mmol/L and 0 5mmol/L). The inhibition rate of tyrosinase was higher than that of hydroquinone (0 5 mmol/L) (P < 0 05). Conclusion Aloin, cinnamic acid and matrine can down-regulate tyrosinase activity. Matrine belongs to non-competitive inhibition of tyrosinase, and aloin and cinnamic acid are mixed inhibitors.