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目的 探讨帕金森病病程中多巴胺D2受体的活性变化及其规律。方法 在建立6-羟基多巴胺毁损的帕金森病大鼠模型基础上,应用放射配基结合分析法结合Scanchard作图,测定不同时间点模型大鼠及对照大鼠尾壳核多巴胺D2受体的最大结合容量(Bmax)和平衡解离常数(KD)。结果 大鼠模型毁损侧尾壳核多巴胺D2受体Bmax显著升高,而KD值显著降低,在1个月时达到高峰,受体的亲合力显著增高。结论 帕金森病大鼠模型毁损侧尾壳核存在D2受体上行调节,D2受体明显超敏。
Objective To investigate the changes of dopamine D2 receptor activity and its regulation in the course of Parkinson’s disease. Methods Based on the establishment of 6-hydroxydopamine-lesioned Parkinson’s disease rat model, radioligand binding assay and Scanchard mapping were used to determine the maximal dopamine D2 receptors in the caudate putamen of model rats and control rats at different time points Binding capacity (Bmax) and equilibrium dissociation constant (KD). Results The Bmax of dopamine D2 receptor in caudal putamen increased significantly in rat model, while the KD value decreased significantly, reaching the peak at 1 month and the affinity of receptor increased significantly. Conclusion The upregulation of D2 receptors on the damaged caudate putamen in Parkinson ’s disease rat model, D2 receptor was significantly hypersensitive.