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目的 探讨心脏停搏液中钙离子 ( Ca2 +)浓度对离体未成熟大鼠心肌的影响 .方法 采用 SD幼鼠 ( 18～ 2 1d)离体工作心模型 ,14℃低温缺血 12 0 min,缺血前分别以 Ca2 +浓度为 0 ,0 .8,1.0 ,1.2 ,1.4和 2 .4mmol.L- 1的 St.Thomas’ 停搏液单次灌注 ,观察记录缺血再灌注后心功能的恢复百分比及心肌酶 CPK的漏出量 .结果 再灌注 30 min后 ,各组主动脉流量恢复比依次 0 ,74%± 4% ,80 %± 3% ,70 %±3% ,5 7%± 5 %和 46 %± 4% ,以 Ca2 +浓度为 1.0 mmol.L- 1组最好 ,与 1.2 mmol.L- 1 组相比有显著差异 ( P<0 .0 5 ) .心肌酶 CPK漏出量分别为 ( 312 .8± 15 .3) ,( 47.1± 6 .0 ) ,( 33.5± 7.2 ) ,( 46 .7± 5 .9) ,( 5 3.6± 8.3)和 ( 72 .4± 9.1) nkat.g- 1 .结论 本结果提示停搏液中 Ca2 +浓度对未成熟心肌的影响呈钟形量—效曲线 ,以 1.0 mmol.L- 1时保护效果最佳 ,无钙停搏液可引起钙反常 ,对未成熟心肌是有害的
Objective To investigate the effects of calcium (Ca2 +) concentration in cardioplegic solution on myocardial cells in isolated immature rats.Methods SD rat hearts (18 ~ 21 days) Before ischemia, St. Thomas’ cardioplegia solution with Ca2 + concentrations of 0, 0.8, 1.0, 1.2, 1.4 and 2.4 mmol.L-1 respectively was used to observe the cardiac function after ischemia-reperfusion Recovery rate and CPK leakage.Results After 30 min of reperfusion, the recovery of aortic flow in each group was 0, 74% ± 4%, 80% ± 3%, 70% ± 3%, 57% ± 5% and 46% ± 4%, respectively, with Ca2 + concentration of 1.0 mmol.L-1 group was the best, compared with 1.2 mmol.L-1 group there was significant difference (P <0.05) (312.8 ± 15.3), (47.1 ± 6.0), (33.5 ± 7.2), (46.7 ± 5 .9), (53.6 ± 8.3) and (72.4 ± 9.1 ) nkat.g- 1. Conclusions The results suggest that the effect of Ca2 + concentration in cardioplegia on immature myocardium is bell-shaped dose-response curve, the best protective effect at 1.0 mmol.L-1, calcium-free cardioplegia Can cause abnormal calcium, is harmful to immature myocardium