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以BALB/c小鼠为研究对象,用60Coγ射线进行11.5Gy的腹部一次照射,照后1—3h采用局部肠腔扩张注入法给小鼠空肠肠腔内注入正常大鼠小肠核糖核酸(Ribonucleicacid,RNA),照后1、3、5d取全小肠,制备肠粘液,测定免疫球蛋白分泌型免疫球蛋白A(SecretedimmunoglobulinA,sIgA)含量;取空肠段,观察肠绒毛形态变化;取血,测定内毒素含量;取肠系膜淋巴结,测定细菌移位率。探讨小肠RNA对γ射线照射后小鼠肠粘膜屏障的保护作用。结果表明,小肠RNA可明显提高受照后小鼠肠粘液中免疫球蛋白sIgA的含量(p<0.01),减轻肠绒毛的萎缩及塌陷,改善肠粘膜形态结构,降低肠系膜淋巴结细菌移位率和血中内毒素含量。以上结果显示,小肠RNA对γ线照射后的小鼠肠粘膜屏障有明显的保护作用,能抑制肠道细菌移位和肠内内毒素的吸收。
BALB / c mice were used as experimental subjects, and irradiated with 11.5 Gy of 60Co γ-ray once a day for 1 to 3 hours. Local rat intestinal jejunal intestine was infused with normal rat intestinal RNA (Ribonucleic acid, The total intestine was taken at 1, 3 and 5 days after irradiation to prepare intestinal mucosa. The content of sIgA in serum was determined. The jejunum was removed and the morphology of intestinal villus was observed. Toxin content; take mesenteric lymph nodes, bacterial translocation was measured. To investigate the protective effect of small intestine RNA on intestinal mucosal barrier in mice after γ-ray irradiation. The results showed that small intestine RNA significantly increased immunoglobulin sIgA content (p <0.01) in intestinal mucus of irradiated mice, relieved intestinal villi shrinkage and collapse, improved intestinal mucosa morphology and structure, reduced mesenteric lymph node bacterial translocation rate and Endotoxin content in blood. The above results show that small intestine RNA has a significant protective effect on the intestinal mucosal barrier of mice after γ-ray irradiation, and can inhibit intestinal bacterial translocation and intestinal endotoxin absorption.