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目的：了解ｐ５３基因突变与埃伯斯坦－巴尔病毒（ＥＢＶ）相关性鼻咽部Ｔ细胞淋巴瘤（ＮＰＴ）的关系。方法：应用免疫组织化学方法（ＳＡＢＣ），原位杂交（ＩＳＨ），ＰＣＲ－ＳＳＣＰ对２６例确诊为鼻咽部Ｔ细胞淋巴瘤的病例进行ｐ５３蛋白及基因的检测。结果：ＥＢＶ编码的小ＲＮＡ探针（ＥＢＥＲ）１７／２６例阳性（６５４％）；ｐ５３蛋白１３／２６例阳性（５０％）；对其中１２例进行ｐ５３外显子５，７，８的ＰＣＲ－ＳＳＣＰ检测，发现ｐ５３突变７／１２例（５８３％）阳性，ＥＢＶ阳性组５／８例阳性（６２５％），阴性组２／４例（５０％）阳性，外显子５突变４／１２例（３３３％）阳性，外显子７突变１／１２例（８３％）阳性，外显子８突变３／１２例（２５％）阳性，其中１例同时发生５，８两个外显子突变；ＥＢＥＲ／ｐ５３蛋白表达同时发生有１１例，不发生７例，ＥＢＥＲ＋／ｐ５３－６例，ＥＢＥＲ－／ｐ５３＋２例；ＥＢＥＲ表达（ＩＳＨ）与ｐ５３基因突变（ＰＣＲ－ＳＳＣＰ）同时发生５例，不发生２例，ＥＢＥＲ＋／ｐ５３－（ＰＣＲ－ＳＳＣＰ）３例，ＥＢＥＲ－／ｐ５３＋（ＰＣＲ－ＳＳＣＰ）２例；以ｐ５３蛋白检测为标准，ｐ５３基因突变（ＰＣＲ－ＳＳＣＰ?
Objective: To investigate the relationship between p53 gene mutation and EBV-associated nasopharyngeal T-cell lymphoma (NPT). Methods: Twenty-six cases of nasopharyngeal T-cell lymphoma were detected by immunohistochemistry (SABC), in situ hybridization (ISH) and PCR-SSCP. RESULTS: 17/26 EBV-encoded small RNA probes (EBRs) were positive (65.4%) and 13/26 (50%) of p53 proteins were positive. Exon 5 of p53 PCR-SSCP showed that p53 mutation was found in 7/12 cases (583%) positive, 5/8 cases positive (625%) in EBV positive group and 2/4 cases (50%) positive in EBV positive group 4/12 cases (333%) were positive in sub-5 mutation, 1/12 cases (83%) were positive in exon 7 mutation, 3/12 cases (25%) were positive in exon 8 mutation, of which 1 case There were 11 cases of EBER / p53 protein expression in 7 cases, EBER + / p53 in 6 cases, EBER- / p53 + 2 cases; EBER expression (ISH) and p53 gene mutation (PCR-SSCP) occurred simultaneously in 5 cases, 2 cases did not occur, EBER + / p53- (PCR- SCP) 3 embodiment, EBER- / p53 + (PCR-SSCP) 2 cases; standard to detect p53 protein, p53 gene mutations (PCR-SSCP?