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目的研究不同脂肪酸构成对小鼠血脂代谢、炎症和氧化应激及内皮细胞功能的影响。方法雄性KM小鼠随机分为5组,分别喂饲正常对照饲料、猪油高脂饲料和n-6/n-3多不饱和脂肪酸(PUFA)比值为1∶1、5∶1、20∶1的高脂饲料5周,比较各组小鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、白介素-6(IL-6)、丙二醛(MDA)、超敏C反应蛋白(hsCRP)、肿瘤坏死因子(TNF-α)、脂质过氧化物(LPO)、8-异前列腺素F2α(8-isoPGF2α)、氧化型低密度脂蛋白(ox-LDL)、游离脂肪酸(FFA)、选择素(ES)和血管性血友病因子(v WF)的含量变化。结果猪油组LDL-C和非高密度脂蛋白胆固醇(non-HDL-C)水平显著高于其他各组(P<0.05)。n-6/n-3 PUFA 1∶1组和5∶1组血清TG、TC水平显著低于猪油组(P<0.05)。20∶1组血清FFA水平显著高于1∶1组和5∶1组(P<0.05)。1∶1组和5∶1组血清炎症因子和氧化应激指标及ES水平均显著低于猪油组和20∶1组(P<0.05)。5∶1组血清v WF水平显著低于猪油组和20∶1组(P<0.05)。结论与猪油和高n-6/n-3 PUFA比值高脂饲料相比较,低n-6/n-3PUFA比值高脂饲料可改善小鼠脂代谢、炎症与氧化应激和内皮细胞功能。
Objective To study the effects of different fatty acid composition on blood lipid metabolism, inflammation, oxidative stress and endothelial cell function in mice. Methods Male KM mice were randomly divided into 5 groups and fed with normal diet respectively. The ratio of lard high fat diet to n-6 / n-3 polyunsaturated fatty acid (PUFA) was 1: 1, 5: 1 and 20: 1 high fat diet for 5 weeks. The levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL- IL-6, MDA, hsCRP, TNF-α, LPO, 8-iso-prostaglandin F2α 8-isoPGF2α, ox-LDL, free fatty acid (FFA), selectin (ES) and von Willebrand factor (vWF) Results The levels of LDL-C and non-HDL-C in lard group were significantly higher than those in other groups (P <0.05). The TG and TC levels of n-6 / n-3 PUFA 1: 1 group and 5: 1 group were significantly lower than those of lard oil group (P <0.05). Serum FFA levels in the 20: 1 group were significantly higher than those in the 1: 1 and 5: 1 groups (P <0.05). Serum inflammatory factors and oxidative stress indexes and ES levels in 1: 1 group and 5: 1 group were significantly lower than those in lard group and 20: 1 group (P <0.05). The level of vWF in 5: 1 group was significantly lower than that in lard group and 20: 1 group (P <0.05). Conclusion Compared with lard and high n-6 / n-3 PUFA ratio high-fat diet, low n-6 / n-3 PUFA ratio high fat diet can improve lipid metabolism, inflammation and oxidative stress and endothelial cell function in mice.