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本文讨论了用纯化的白喉类毒素(DT)和破伤风类毒素(TT)与蛋黄或药用植物播娘蒿(LRS)制成合剂,给动物和人口服免疫后比较了它们的安全性及中和抗体应答.DT(500Lf(?)ml)和TT(250Lf/ml)口服前用磷酸盐缓冲盐水(PBS)稀释,加新鲜蛋黄或LRS室温搅拌混合30分钟.为消除胃肠道消化酶对类毒素的蛋白酶活性,口服前给动物静脉注射1ml蛋白酶抑制剂抑肽酸,志愿者则口服相同量抑肽酶.
This article discusses a combination of purified diphtheria toxoid (DT) and tetanus toxoid (TT) with egg yolk or the medicinal plant Artemisia annua L. (LRS) to compare their safety against oral administration to animals and humans, (500Lf (?) Ml) and TT (250Lf / ml) were diluted with phosphate buffered saline (PBS) before oral administration, freshly mixed with fresh egg yolk or LRS at room temperature for 30 minutes to eliminate gastrointestinal tract digestive enzymes To the toxin-like protease activity, 1 ml of the protease inhibitor pepstatin was intravenously administered to animals prior to oral administration, while the volunteers orally administered the same amount of aprotinin.