目的:建立美味猕猴桃茎的HPLC指纹图谱。方法:采用HPLC法,色谱柱Thermo Hypersil BDS C18柱(250 mm×4.6 mm,5μm),流动相以乙腈-0.1%磷酸溶液梯度洗脱,柱温20℃,检测波长296 nm,流速1.0 mL/min,进样量20μL。结果:建立了美味猕猴桃茎药材的指纹图谱,并检测了不同产地16批美味猕猴桃茎药材,标定了28个共有峰,相似度较高,各色谱峰分离较好,达到中药指纹图谱研究的技术要求。结论:该方法准确可靠,为更好地控制药材内在质量提供了科学依据。 Objective: To establish HPLC fingerprinting of kiwi fruit stem. METHODS: The mobile phase was eluted with a gradient of acetonitrile-0.1% phosphoric acid solution on a Thermo Hypersil BDS C18 column (250 mm × 4.6 mm, 5 μm) using a HPLC method with a detection wavelength of 296 nm and a flow rate of 1.0 mL / min, injection volume 20μL. Results: The fingerprints of kiwifruit stalks were established and 16 batches of kiwifruit stalks were tested. The common peaks were calibrated and the similarity was high. The peaks of the chromatographic peaks were separated well and the technology of fingerprinting Claim. Conclusion: The method is accurate and reliable, providing a scientific basis for better control of the intrinsic quality of medicinal materials.