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目的:建立对福美司坦药物来源的检测方法,并进行人体代谢的检测研究。方法:采用酶解、提取、高效液相色谱纯化等方法对尿样进行预处理,应用同位素比质谱仪测定相关组分的同位素比(13C/12C,δ值),建立检测方法。本实验中招募2名男性健康受试者,每位受试者口服福美司坦后收集尿样,应用所建立的检测方法对尿样进行分析,探讨福美司坦在人体内代谢过程中的检测规律。结果:建立的分析方法精密度及重现性RSD均小于1%。受试者尿样中福美斯坦的δ值在服药后迅速降低,并在27小时内保持在约为-31‰。内源性雄性激素代谢物雄酮androsterone和本胆烷醇酮etiocholanolone的δ值无显著性变化。结论:在兴奋剂检测中确定以福美司坦药物原形为目标化合物进行来源分析,建立了检测福美斯坦兴奋剂的同位素比质谱分析方法。
OBJECTIVE: To establish a method for the determination of the source of Fomestane, and to study the detection of human metabolism. Methods: Urine samples were pretreated by enzymatic hydrolysis, extraction and HPLC. The isotope ratios (13C / 12C and δ values) of related components were determined by isotope ratio mass spectrometry. In this study, two male healthy subjects were enrolled. Each patient was enrolled in urine samples after oral administration of formate, and the urine samples were analyzed by using the established detection method to explore the detection of formestane during the metabolic process in the human body law. Results: The precision and reproducibility of the established analytical methods were all less than 1%. The values of formestane in the urine of the subjects decreased rapidly after taking the drug and remained at about -31 ‰ within 27 hours. There was no significant change in the δ values of androsterone, an endogenous androgen metabolite, and etiocholanolone. Conclusion: It is determined that the prototype of fomeistemine is taken as the target compound for doping analysis, and the isotope ratio mass spectrometry method for the determination of fomestein stimulant is established.