论文部分内容阅读
目的:观察姜黄素对人肝星状细胞LX-2凋亡与Collagenα1(Ⅰ)mRNA表达的影响,阐明其保护肝脏及抗纤维化机制。方法:采用人肝星状细胞株LX-2作为研究模型,经不同剂量姜黄素(终浓度为10、20、40、80μmol/L)作用24 h后,应用流式细胞仪Annexin/PI双染方法检测姜黄素对LX-2细胞凋亡的影响;应用RT-PCR检测姜黄素对LX-2细胞Collagenα1(Ⅰ)mRNA表达的影响。结果:姜黄素可显著促进LX-2细胞凋亡,细胞早期凋亡率随姜黄素质量浓度增加而提高,与空白对照组比较,姜黄素20、40、80μmol/L剂量组凋亡率显著升高(P<0.05或P<0.01);同时姜黄素可显著降低LX-2细胞的Collagenα1(Ⅰ)mRNA表达,与空白对照组比较差异显著(P<0.01)。结论:促进肝星状细胞凋亡,抑制其Collagenα1(Ⅰ)的表达是姜黄素抗肝纤维化的部分机制。
Objective: To observe the effects of curcumin on the apoptosis of human hepatic stellate cells LX-2 and the expression of Collagen α1 (I) mRNA and elucidate the mechanism of liver protection and anti-fibrosis. METHODS: Human hepatic stellate cell line LX-2 was used as a research model. After different doses of curcumin (10, 20, 40, and 80 μmol/L) for 24 h, flow cytometer Annexin/PI double staining was used. Methods The effects of curcumin on the apoptosis of LX-2 cells were detected. The effect of curcumin on the expression of Collagen α1 (I) mRNA in LX-2 cells was detected by RT-PCR. RESULTS: Curcumin could significantly promote the apoptosis of LX-2 cells, and the early apoptosis rate increased with the increase of the curcumin concentration. Compared with the blank control group, the apoptotic rate of curcumin at 20, 40, and 80 μmol/L increased significantly. High (P<0.05 or P<0.01); while curcumin can significantly reduce the expression of Collagenα1(I) mRNA in LX-2 cells, which is significantly different from the blank control group (P<0.01). CONCLUSION: The promotion of hepatic stellate cell apoptosis and the inhibition of its expression of Collagen α1 (I) are part of curcumin’s anti-fibrotic mechanism.