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目的对福州市2004年登革热疫情及病原学特征进行分析。方法调查和分析福州市2004年登革热流行病学特点及影响因素;用C6／36细胞分离患者血清标本中病毒,并用单克隆抗体鉴定型别;通过比对核苷酸序列,分析本次疫情可能的传染源。结果福州市2004年9月中旬起至10月底发生一起登革热暴发疫情,所确认的93例病例消长情况基本与当地的蚊媒消长情况相一致。从病例的10份血清标本中分离出6株病毒,经单克隆抗体鉴定均为登革热病毒1型,病毒基因的核苷酸序列比对证明同源性与东埔寨分离株(DENV-1／KHM／2001;GenBank Accession No．L0904278)最为接近。结论福州市2004年登革热疫情为感染登革1型病毒所引起;疫情的发生主要为输入性病例流行引起。
Objective To analyze the epidemic and etiological characteristics of dengue fever in Fuzhou in 2004. Methods The epidemiological characteristics and influencing factors of dengue in 2004 in Fuzhou were investigated and analyzed. The virus in the serum samples of patients was separated by C6 / 36 cells and identified by monoclonal antibody. By comparing the nucleotide sequences, the epidemic situation was analyzed The source of infection. Results A total outbreak of Dengue Fever occurred in Fuzhou from mid-September 2004 to the end of October 2004. The confirmed 93 cases were basically consistent with the growth and decline of mosquito vectors. Six strains of viruses were isolated from 10 serum samples of the case and were all identified as dengue virus type 1 by monoclonal antibody. The homology of the nucleotide sequence of the virus gene was verified by DENV-1 / KHM / 2001; GenBank Accession No. L0904278). Conclusion The epidemic situation of dengue in Fuzhou was caused by the infection of dengue type 1 virus in 2004; the epidemic was mainly caused by the prevalence of imported cases.