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在基础饲料中分别添加3个水平(0.5%、1%、2%)的中草药制剂煎液制成3种试验饲料,同时以基础饲料为对照。采用连续投喂的方法,饲喂对虾30d,分别测定了第5,10,15,20,25,30d血淋巴中的酚氧化酶、过氧化物酶、抗菌活力等免疫因子活性,并对血清中蛋白进行定量。试验结束后,试验组及对照组存活率都很高(95%￣98%),各试验组间无差异(P>0.05)。饲喂1%、2%中草药组增重率、增长率都显著高于(P<0.01)对照组。试验组酚氧化酶活力、过氧化物酶活力、抗菌活力都显著高于对照组(P<0.01)。试验结果表明,中草药制剂能有效促进对虾的生长及提高免疫因子的活性。饲养试验结束7d后,腹腔注射副溶血弧菌进行攻毒试验,在8d内,对照组的死亡率显著高于试验组(P<0.05),试验组免疫保护率为50%￣65%,以2%中草药组最为显著。本试验结果表明:中草药制剂可以促进凡纳滨对虾的生长,增强免疫力和抗病力;从免疫水平和生长水平综合考虑,添加量为1%￣2%为宜。
In the basal diet, three levels (0.5%, 1%, 2%) of Chinese herbal medicine decoction were added to make three kinds of test feed, and the basic feed was used as control. Adopting continuous feeding method, the shrimp were fed with shrimp for 30 days, and the activities of phenoloxidase, peroxidase, antimicrobial activity and other immune factors in hemolymph on the 5th, 10th, 15th, 20th, Protein in the quantitative. After the test, the survival rate of the experimental group and the control group were very high (95% ~ 98%), there was no difference between the experimental groups (P> 0.05). The weight gain rate and the growth rate of 1%, 2% Chinese herbal medicine group were significantly higher than those of the control group (P <0.01). The activities of phenoloxidase, peroxidase and antibacterial activity in the experimental group were significantly higher than those in the control group (P <0.01). The experimental results show that Chinese herbal medicine can effectively promote the growth of shrimp and increase the activity of immune factors. Seven days after the end of feeding experiment, Vibrio parahaemolyticus was injected intraperitoneally for challenge test. Within 8 days, the mortality of the control group was significantly higher than that of the experimental group (P <0.05). The immune protection rate of the experimental group was 50% ~ 65% 2% Chinese herbal medicine group the most significant. The results show that: Chinese herbal medicine can promote the growth of Litopenaeus vannamei, enhance immunity and disease resistance; from the immune level and the level of growth, the amount of 1% ~ 2% is appropriate.