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AIM: To differentiate the electrophysiological characteristics of somatic action potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those from slices of intact Juv and adult rat nodose ganglia. METHODS: For isolated cell recordings nodose ganglia from 3 - 8 d old Neo and 4 weeks old Juv rats were dissociated using trypsin and collagenase, respectively. Nodose ganglia slices with attached vagus were prepared using a sequential treatment of collagenase and trypsin for both Juv and adult rats. Conduction velocity (CV) was collected by vagal stimulation. Whole-cell patch was applied for somatic AP recordings. RESULTS: (1) 281 NSN from both isolated cells and nodose slices were studied. Across all age groups, there was no difference observed among either C- or A-types. The difference between C- and A-type was significant. (2) Neurons exhibiting AP with prominent repolarization hump, broader APD50( >2.0 ms), upstroke velocity at the point of APD50(UVAPD50) and downstroke velocity at th
AIM: To differentiate the electrophysiological characteristics of somatic action potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those from slices of intact Juv and adult rat nodose ganglia. METHODS: For isolated cell recordings nodose ganglia from 3 - 8 d old Neo and 4 weeks old Juv rats were dissociated using trypsin and collagenase, respectively. Nodose ganglia slices with attached vagus were prepared using a sequential treatment of collagenase and trypsin for both Juv and adult rats. Conduction velocity (CV) was collected by vagal stimulation. Whole-cell patch was applied for somatic AP recordings. RESULTS: (1) 281 NSN from both isolated cells and nodose slices were studied. Across all age groups, there was no difference observed among either C- or A-types. The (2) Neurons exhibiting AP with prominent repolarization hump, broader APD50 (> 2.0 ms), upstroke velocity at the point of APD50 (UVAPD50) and downstr oke velocity at th