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为探讨庚型肝炎病毒(GBV-C/HGV)感染有无致病性,利用GBV-C/HGV不同基因区序列设计并优选出5套引物,建立起灵敏、特异的HGV逆转录套式聚合酶链反应(RT-nPCR)检测方法.应用此方法对临床上诊断为乙型肝炎及慢性丙型肝炎的患者,按照其血清中HGV RNA的阳性与否分组,并进行病例对照研究.结果显示,单独HBV或HCV感染的肝炎患者与混合HGV感染的肝炎患者的血清肝功能异常指标无显著性差异(P>0.05).因此,病毒性肝炎患者混合感染HGV后,未发现加重HBV或HCV感染的肝功能异常程度,不支持HGV有明显的嗜肝致病性.
To investigate the presence or absence of pathogenicity of hepatitis B virus (GV-C / HGV) infection, 5 sets of primers were designed and optimized based on the sequences of different regions of GBV-C / HGV to establish a sensitive and specific reverse transcriptase-mediated polymerization Enzyme-linked immunosorbent assay (RT-nPCR) was used to detect patients with clinically diagnosed hepatitis B and chronic hepatitis C according to their serum HGV RNA positive or not, and case-control study was carried out. (P> 0.05) .Therefore, there was no significant difference (P> 0.05) in the index of serum liver function in hepatitis patients with HBV or HCV infection alone or in patients with mixed HGV infection.Therefore, no aggravation of HBV or HCV infection was found after mixed infection of HGV patients with viral hepatitis The degree of abnormal liver function does not support HGV has a significant liver pathogenic.