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在镀银电极的压电石英晶体表面涂覆甲基丙烯酸甲酯－甲基丙烯酸羟乙酯共聚物涂层，经ＣＮＢｒ活化后固定抗体，用石英晶体微天平技术监测ＩｇＭ抗原在压电石英晶体上的吸附与解吸过程，研究了免疫反应体系ＩｇＭ－抗人ＩｇＭ的反应动力学．结果表明：ＩｇＭ在压电石英晶体上的键合行为很好地符合Ｌａｎｇｍｕｉｒ吸附方程，免疫反应的结合常数Ｋａｓｓ为７．１０×１０￣７（ｍｏｌ／Ｌ）￣（－１）．在抗原过量情况下为一级反应。计算了ＩｇＭ免疫反应的活化能Ｅａ和不同温度下的反应速率常数ｋｆ．
In the silver plated electrode piezoelectric quartz crystal surface coated with methyl methacrylate - hydroxyethyl methacrylate copolymer coating by CNBr activation after the fixed antibody, with quartz crystal microbalance monitoring IgM antigen in the piezoelectric quartz crystal On the adsorption and desorption process, the immune reaction system IgM-anti-human IgM reaction kinetics. The results show that the bonding behavior of IgM on the piezoelectric quartz crystal well accords with the Langmuir adsorption equation. The binding constant Kass of the immunoreaction is 7.10 × 10 ~ (mol / L) ~ (-1). In the case of excessive antigen level response. The activation energy Ea of IgM immune response and the reaction rate constant kf at different temperatures were calculated.