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为了探讨白细胞介素 -1I型受体 ( IL-1RI)在癫痫发病中的作用 ,本实验采用免疫组织化学方法观察了致痫大鼠行为改变与脑内 IL-1RI表达变化。结果发现 ,侧脑室注射白细胞介素 -1β( IL-1β)后再注射阈下剂量谷氨酸钠 ,可导致动物痫样发作 ,其大脑皮质及海马锥体细胞层 IL -1RI免疫反应阳性细胞数量较对照组明显增多 ,免疫反应增强 ;如先注射白细胞介素 1受体拮抗剂 ( IL-1ra)、再注射 IL-1β和阈下剂量谷氨酸钠 ,则动物无痫样发作 ,且大脑皮质及海马锥体细胞层 IL-1RI免疫反应阳性细胞数量较注射 IL -1β和阈下剂量谷氨酸钠组减少 ,免疫反应着色减弱。结果提示 ,IL -1β有明显促进谷氨酸钠致痫的作用 ,IL-1RI可能参与致痫过程 ,IL-1ra具有抗痫效应。
In order to investigate the role of interleukin-1 receptor (IL-1RI) in the pathogenesis of epilepsy, we used immunohistochemistry to observe the changes of behavior and the expression of IL-1RI in the epileptic rats. The results showed that intracerebroventricular injection of interleukin-1β (IL-1β) followed by subthreshold dose of glutamate could lead to epileptiform attack, IL-1RI immunoreactive cells in the cerebral cortex and hippocampal pyramidal cells Compared with the control group, the number increased significantly and the immune response increased. For example, IL-1ra was injected first and then IL-1β and subthreshold dose of sodium glutamate, the animals had no seizures, and The number of IL-1RI-immunoreactive cells in cerebral cortex and hippocampal pyramidal cells was decreased compared with that of IL-1β and subthreshold dose of glutamate, and the immunoreaction staining was weakened. The results suggest that IL-1β significantly promote the role of sodium glutamate-induced epilepsy, IL-1RI may be involved in the process of epilepsy, IL-1ra has anti-epileptic effect.