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为明确枣疯病植原体越冬后向新生组织的转移扩散特点,从而指导枣疯病预防和控制,本研究采用PCR和DAPI荧光显微技术,比较枣疯病植原体向枣树不同类型新生组织的转移情况。结果表明:室内水培的疯枣枝在萌芽30d后,新生叶片可以检测到植原体;自然栽培枣树的枣股新生叶片生长发育至24d出现植原体;枣树根蘖在萌芽后12d即可检测到植原体。嫁接试验表明,嫁接于染病冬枣砧木上的易感品种接穗‘唐星’,‘阜星’,其萌发的新生幼叶在第34天即可检测到植原体,而抗病品种‘星光’接穗在第119天才检测到植原体。由此可见,枣疯病植原体在地上和地下部分均可以越冬,越冬的植原体向枣树不同类型和不同品种新生组织中的转移扩散速度存在差异,由快到慢为根蘖>自然生枝条>离体水培枝条>嫁接接穗。
In order to clarify the characteristics of juvenile Phytoplankton (Phytoplankton) metastasis and proliferation to the new tissue after overwintering, and to guide the prevention and control of jujube disease, we used PCR and DAPI fluorescence microscopy to compare the different types of juvenile The transfer of the situation. The results showed that the phytoplankton could be detected in the leaves of wild jujube by the method of in vitro hydroponics after 30 days of germination. The growth of jujube leaves of naturally cultivated jujube tree appeared to be phytoplankton 24 days later. The root tiller of jujube tree could be 12 days after germination Phytoplasma was detected. The grafting experiments showed that the susceptible cultivar “Tangxing” and “Fuxing”, which were susceptible to the infected winter jujube rootstocks, could detect phytoplasmas on the 34th day, while the resistant varieties ’Starlight’ scion Phytoplasma was detected on day 119. Thus, jujube broom Phytoplankton overwintering in the ground and underground parts, wintering phytoplankton jujube different types and different varieties of new tissue transfer rate of diffusion differences, from fast to slow for the root tiller> natural Branches> In vitro hydroponics branches> Grafted scion.