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目的报告《建立广州生物库进行慢性重大疾病研究》过去十年的研究进展和今后十年的研究展望。方法选择≥50岁的30 518名有广州市户籍的居民,收集其个人背景资料、职业接触、居住和生活环境与习惯、营养摄入及个人与家族史等详细资料,并作体格检查,血生化检验、尿柯铁宁及心电图、X线胸片与肺功能检测等,同时储存其白细胞与血浆在-196℃液态氮库内使其永生化;定期重复体检随访及电话随访并建立亚队列进行基因、生活方式与职业环境等因素对慢性重大疾病影响的研究。计数资料采用χ2检验,计量资料符合正态分布的采用t检验,不符合正态分布的采用秩和检验,以双侧P<0.05为差异有统计学意义。结果 (1)建立液态氮生物库、条码识别技术与Fico11-Pague分离细胞室及分子流行病学研究室的管理系统;(2)分三个阶段收集30 518名研究对象建立生物库中老年队列;(3)基线资料分析表明,“三高”凸显,男女血压高值分别为24.0%和17.8%,高血压患病率分别为45.3%和41.3%,χ2值为39.89,P<0.001;血糖高值和糖尿病患者男性分别为11.1%和12.0%,略高于女性的9.9%和12.6%,χ2值分别为9.00和2.04,P=0.191和0.293,差异无统计学意义;而高脂血症更为明显,检出率则女性明显高于男性;(4)中央型肥胖、超重和全身性肥胖以及代谢综合征也呈现女性高于男性;短期记忆受损则男性为13.9%,稍高于女性的12.7%,χ2值为8.05,P=0.005;(5)发现不良的生活方式如吸烟、二手烟、饮酒、缺乏体力活动、不良睡眠状态及打鼾对健康的影响,经历不同社会经济环境(生命历程)成长发育对健康的影响,研究已发表论文80多篇;(6)第一次重复体检和电话随访已于2012年12月结束,除606名研究对象因户口迁出或身份证有误无法确定外,成功随访率达98.0%,已知1 801例死亡,随访资料仍在处理和分析中。结论既往十年《广州生物库中老年队列研究》项目已建立标准化的研究平台及完成第一次重复体检与电话随访,未来十年将继续重复体检为主的纵向随访及建立亚队列进行基因、职业与环境因素及生活方式对慢性重大疾病影响的研究,不断向深度和广度发展。
Objective To report the research progress in the past decade and the prospect of research in the “Establishment of Guangzhou Biobank for the Study of Chronic and Major Diseases”. Methods A total of 30 518 residents aged 50 or above who had a census register in Guangzhou were recruited and their personal background information, occupational exposure, living and living environment and habits, nutritional intake and personal and family history were collected. Physical examination, blood test Biochemical tests, urinary Ke Tiening and ECG, chest X-ray and lung function tests, while its white blood cells and plasma stored at -196 ℃ liquid nitrogen to make it immortalized; regular follow-up and telephone follow-up and the establishment of sub-queue gene , Lifestyle and occupational environment and other factors on the impact of chronic diseases. Count data using χ2 test, measurement data in line with the normal distribution using t test, does not meet the normal distribution using rank sum test to bilateral P <0.05 was considered statistically significant. Results (1) The establishment of liquid nitrogen biological pool, barcode recognition technology and Fico11-Pague isolation cell and molecular epidemiology laboratory management system; (2) The collection of 30 518 research objects in three stages to establish the biological library in the elderly cohort ; (3) Baseline data analysis showed that the high blood pressure of men and women were respectively 24.0% and 17.8%, the prevalence of hypertension was 45.3% and 41.3% respectively, the value of χ2 was 39.89, P <0.001 ; High blood sugar and diabetes were 11.1% and 12.0% of men, slightly higher than the female 9.9% and 12.6%, χ2 values were 9.00 and 2.04, P = 0.191 and 0.293, the difference was not statistically significant; and high fat (4) Central obesity, overweight and generalized obesity, and metabolic syndrome also showed a higher prevalence of women than men; short-term memory impairment was 13.9% for males, slightly higher than men Higher than female 12.7%, χ2 = 8.05, P = 0.005; (5) found that poor lifestyles such as smoking, secondhand smoke, alcohol consumption, lack of physical activity, poor sleep status and the impact of snoring on health experience different socio-economic Environment (life course) growth and development of health effects, research has published more than 80 papers; (6) the first Repeated medical examinations and phone follow-up were completed in December 2012. The successful follow-up rate was 98.0% with 1,601 deaths and the follow-up data was still in place except for 606 subjects who were unable to confirm the account relocation or ID card errors Processing and analysis. Conclusion In the past ten years, “Guangzhou Elderly Cohort Collaborative Research” project has established a standardized research platform and completed the first repeat medical examination and telephone follow-up. In the next ten years, we will continue to repeat the medical examination based longitudinal follow-up and establish sub-cohort to carry out gene, Occupational and environmental factors and lifestyle impact on chronic major diseases, continue to develop to depth and breadth.