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目的 :通过骨髓细胞移植对心肌梗死大鼠模型血管内皮生长因子及其受体表达的影响 ,探讨骨髓细胞移植改善缺血心脏功能的可能机制。方法 :利用左前降支冠状动脉结扎术制备大鼠心肌梗死模型 ,然后行异体骨髓细胞移植 ;分别于术后 1d ,3d ,7d ,14d和 2 8d取材 ;利用免疫荧光和RT -PCR技术分析细胞移植对血管内皮生长因子 (VEGF)及其受体 (Flk 1)表达的影响和移植细胞的分化情况 ;通过免疫组化计算血管数量 ;应用血流动力学检测大鼠心脏功能在术后各时间点的变化。结果 :VEGF和Flk 1在移植组动物的心肌梗死区残存细胞、梗死周边区细胞以及部分移植细胞内表达。移植组VEGF和Flk 1的mRNA表达明显高于对照组 ,分别于术后 3d和 14d达到高峰 ,以后逐渐减弱。术后 7d ,14d和 2 8d移植组血管数量较同期对照组明显增加 ,移植组心脏功能较对照组明显改善。术后 14d可在部分移植细胞中检测到心肌细胞或血管内皮细胞特异性蛋白的表达。结论 :骨髓细胞移植通过上调移植细胞及受者内源性VEGF和Flk 1的表达 ,促进血管新生 ,进而改善缺血心脏功能。
OBJECTIVE: To investigate the effect of bone marrow cell transplantation on the expression of vascular endothelial growth factor and its receptor in rat model of myocardial infarction and to explore the possible mechanism of bone marrow cell transplantation in improving ischemic cardiac function. Methods: The model of myocardial infarction was established by ligation of the left anterior descending coronary artery and then allogeneic myeloid cells were transplanted. The cells were harvested at 1d, 3d, 7d, 14d and 28d after operation respectively. Cells were analyzed by immunofluorescence and RT-PCR Transplantation of vascular endothelial growth factor (VEGF) and its receptor (Flk 1) expression and the differentiation of transplanted cells; by immunohistochemistry to calculate the number of blood vessels; use of hemodynamics to detect rat heart function at various times after surgery Point changes. Results: VEGF and Flk 1 were expressed in the myocardial infarction area, the peripheral infarction area and some of the transplanted cells in the transplantation group. The mRNA expression of VEGF and Flk 1 in the transplantation group was significantly higher than that in the control group, reaching the peak at 3d and 14d respectively and then gradually weakened. The numbers of blood vessels in the graft group at 7d, 14d and 28d after operation were significantly increased compared with the control group at the same period, and the cardiac function in the transplantation group was significantly improved compared with the control group. At 14 days after operation, the expression of cardiomyocytes or vascular endothelial cell specific protein was detected in some of the transplanted cells. CONCLUSION: Bone marrow cell transplantation can promote angiogenesis by up-regulating the expression of endogenous VEGF and Flk-1 in transplanted cells and recipients, thus improving ischemic cardiac function.