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目的:研究成年大鼠肌源性干细胞纯化培养方法以及与胶原海绵的细胞相容性,探讨构建组织工程化肌性吊带的可行性。方法:取成年雌性SD大鼠前肢肱三头肌,用胶原酶和Dispase进行消化,采用差速贴壁的方法纯化肌源性干细胞并与胶原海绵体外复合培养,构建组织工程化肌源性干细胞/胶原海绵复合体。结果:成功地分离培养了成年大鼠的肌源性干细胞,肌源性干细胞在胶原海绵上生长良好、增殖迅速。结论:采用差速贴壁的方法可以纯化肌源性干细胞,肌源性干细胞与胶原海绵有良好的细胞相容性,本实验为构建组织工程化肌性吊带的研究打下基础。
OBJECTIVE: To study the method of purification and culture of myogenic stem cells from adult rats and the cytocompatibility with collagen sponge, and to explore the feasibility of constructing tissue-engineered muscle sling. METHODS: The triceps brachii of adult female SD rats were digested with collagenase and Dispase. The myogenic stem cells were purified by differential adherent method and cultured with collagen sponge in vitro to construct tissue-engineered myogenic stem cells Collagen sponge complex. RESULTS: Muscle-derived stem cells of adult rats were successfully isolated and cultured. Muscle-derived stem cells grew well on collagen sponges and proliferated rapidly. CONCLUSION: Myogenic stem cells can be purified by differential adherent method. Myogenic stem cells and collagen sponge have good cytocompatibility. This study laid the foundation for the construction of tissue-engineered muscle sling.