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目的:研究金鸡菊(Coreopsis tinctoria Nuff.)乙醇提取物对离体血管平滑肌张力影响,同时探讨其作用机制。方法:采用大鼠离体胸主动脉灌流,应用金鸡菊提取物(0.25、0.50、0.75和1.00 mg/ml)记录张力变化,观测其对Sprague Dawley(SD)大鼠离体胸主动脉环的作用,L-NAME及不同钾通道阻滞剂的影响。结果:金鸡菊提取物0.50~1.00 mg/ml对氯化钾(60 mmol/L)和苯肾上腺素(0.3μmol/L)预收缩的血管环具有浓度依赖的舒张作用,对内皮完整和去内皮血管环舒张作用无差异,该舒张作用为非内皮依赖性。在KCl预收缩基础上,加入一氧化氮合酶抑制剂(L-NAME,100μmol/L)和内向整流钾通道阻断剂钾通道阻断剂氯化钡(BaCl2,30μmol/L)均不能抑制金鸡菊提取物提取物的舒血管效应,非选择性钾通道阻断剂(TEA,10mmol/L)、电压依赖性钾通道阻断剂四氨基吡啶(4-AP,1 mmol/L)、ATP敏感钾通道阻断剂(Gli,10μmol/L)及大电导激活钾通道阻断剂(IbTX,100 nmol/L)能抑制金鸡菊提取物提取物对血管的舒张作用。结论:金鸡菊提取物提取物舒张血管的作用具有浓度依赖性,其作用机制可能与激活KATP、BKca和Kv有关。
Objective: To study the effect of ethanol extract of Coreopsis tinctoria Nuff. On the in vitro vascular smooth muscle tension and to explore its mechanism. Methods: The changes of tension were recorded in isolated rat aorta thoracic aorta. The changes of the tension of the isolated rat aorta rings of Sprague Dawley (SD) rats were observed using the extract of Coreopsis chinensis (0.25,0.50,0.75 and 1.00 mg / ml) Effect, L-NAME and different potassium channel blockers. Results: 0.50 ~ 1.00 mg / ml of Coreopsis extract had a concentration-dependent relaxation effect on the precontracted vascular rings of potassium chloride (60 mmol / L) and phenylephrine (0.3 μmol / L) There was no difference in vascular ring relaxation, which is not endothelium-dependent. On the basis of KCl precontraction, the addition of nitric oxide synthase inhibitor (L-NAME, 100μmol / L) and the inward rectifier potassium channel blocker potassium channel blocker barium chloride (BaCl2, 30μmol / L) The vasodilator effects of extracts from Coreopsis sp., Non-selective potassium channel blockers (TEA, 10 mmol / L), voltage dependent potassium channel blockers tetraaminopyridine (4-AP, 1 mmol / L) Sensitive potassium channel blockers (Gli, 10μmol / L) and large conductance activated potassium channel blockers (IbTX, 100 nmol / L) inhibited the vasorelaxation effect of extracts of Coreopsis extract on blood vessels. Conclusion: The effect of extract of Coreopsis extract on vasodilation is concentration-dependent, and its mechanism may be related to the activation of KATP, BKca and Kv.