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在体外用IFN-Y和TNF-。等细胞因子处理B7-1表达阳性的Hepal-6,观察其对小鼠肝癌表面MHC Class Ⅰ和ICAM-1表达的调节作用和对体内外抗肿瘤免疫反应的影响。结果发现:单独B7-1基因转染的Hepal-6的致瘤性消失,但仅能诱导部分细胞毒性和部分保护性免疫反应,而B7-1基因转染与细胞因子处理联合应用则能诱导强烈的细胞毒杀伤活性和完全的抗Hepal-6保护性免疫反应。结论B7-1共刺激分子在小鼠肝癌的表达是激活抗肿瘤免疫所必需的但不充分,而B7基因转染联合IFN-7等细胞因子处理能诱导彻底的抗肿瘤免疫反应。
IFN-γ and TNF- were used in vitro. Cytokines were used to treat Hepal-6 with positive expression of B7-1, and its effect on the regulation of MHC Class I and ICAM-1 expression on mouse liver cancer surface and the effect of anti-tumor immune responses in vitro and in vivo were observed. The results showed that the tumorigenicity of Hepal-6 transfected with B7-1 alone disappeared, but only partial cytotoxicity and partial protective immune responses could be induced, and the combination of B7-1 gene transfection and cytokine treatment could induce Strong cytotoxic activity and complete anti-Hepal-6 protective immune response. Conclusion The expression of B7-1 co-stimulatory molecule in mouse liver cancer is necessary but not sufficient to activate anti-tumor immunity. B7 gene transfection combined with cytokines such as IFN-7 can induce a complete anti-tumor immune response.