论文部分内容阅读
目的:探讨5-氨基酮戊酸(5-ALA)介导的LED-光动力疗法(photodynamic therapy,PDT)联合放疗对人卵巢癌SKOV3细胞的杀伤作用。方法:常氧与乏氧下SKOV3细胞各实验组经不同浓度的5-ALA培养4 h后,由不同功率密度的635nm红光和6 Gy X线照射,48 h后MTT法检测细胞存活率。结果:常氧与乏氧下0.1~0.7 mmol·L-1范围内,5-ALA无细胞毒性与放射增敏性(P>0.05);5-ALA>0.1 mmol·L-1,随着药物浓度与红光功率密度的增加,细胞存活率呈明显的下降趋势;X线与5-ALA-PDT联用,Ra×Rb/Rc比值分别为2.13、1.49,且常氧下联用与顺序无关,乏氧下有关(P<0.01)。结论:常氧与乏氧下X线与PDT联用都是协同作用,但乏氧下联用效果弱于常氧,且先X线照射后PDT可能会是一个更好的联用方式。
Objective: To investigate the killing effect of 5-aminolevulinic acid (5-ALA) -mediated photodynamic therapy (PDT) and radiotherapy on human ovarian cancer SKOV3 cells. Methods: The cultured SKOV3 cells were cultured with 5-ALA at different concentrations for 4 h under both normoxia and hypoxia. Cell viability was measured by MTT assay at 635 nm and 6 Gy X-ray at different power densities. Results: The cytotoxicity and radiosensitivity of 5-ALA in the range of 0.1-0.7 mmol·L-1 under normoxia and hypoxia (P> 0.05) and 5-ALA> 0.1 mmol·L-1 The ratio of Ra × Rb / Rc was 2.13 and 1.49 for X-ray combined with 5-ALA-PDT, respectively, and the correlation between normoxia and sequence was irrelevant , Hypoxia (P <0.01). CONCLUSION: Both normoxia and hypoxia are synergistic effects of X-ray and PDT, but hypoxia combined effect is weaker than normoxia, and PDT may be a better combination mode after X-ray irradiation.