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目的探索应用人工合成高分子聚合材料制备微泡超声造影剂的方法并观察其体外显影效果。方法采用人工合成高分子聚合物乳酸/羟基乙酸共聚物(PLGA)作为成膜材料,通过双乳化法首先制备包裹水滴的PLGA微球,再通过真空冷冻干燥使微球内的水分升华,形成空隙,然后在冷冻干燥室内缓慢冲入氟烷气体,从而制备内含氟烷气体的PLGA微泡超声造影剂高聚显。将一定浓度的高聚显溶液水囊与脱汽水水囊在体外进行超声显影实验,观察其显影效果。结果光镜和扫描电镜观察,采用该法制备的高聚显微泡造影剂呈球形,形态规则,大小均匀,表面光滑;高聚显冻干粉复溶后分散度好;激光测径仪检测其粒径分布窄,平均粒径大小与制备过程中声振仪的设置相关;体外显影效果好。结论采用该方法制备的PLGA微泡超声造影剂高聚显具有大小均匀、显影效果好、抗压性强等特点,同时由于其外壳材料能在体内生物降解,对人体无毒副作用,因而具有广阔的应用前景。
OBJECTIVE: To explore a method for preparing microbubble ultrasound contrast agent by using artificial polymeric materials and to observe its in vitro development effect. Methods Poly (lactic-co-glycolic acid) (PLGA) was synthesized as a membrane-forming material. Firstly, the PLGA microspheres encapsulating water droplets were prepared by double emulsion method. The water was sublimated through vacuum freeze-drying to form voids , And then slowly flushed into the freeze-drying chamber of halothane gas, thereby preparing fluorocarbon gas-containing PLGA microbubble ultrasound contrast agent high polymer significant. A certain concentration of high polymer solution in water sac and dewatering water sac in vitro ultrasonic imaging experiment to observe the development effect. Results The light microscopy and scanning electron microscopy showed that the high contrast microbubble contrast agent prepared by this method was spherical with regular shape, uniform size and smooth surface. The dispersion of high polymer freeze-dried powder after reconstitution was good. The particle size distribution Narrow, the average size of the particle size and preparation process of acoustic vibrometer settings related to in vitro imaging effect. Conclusion PLGA microbubbles ultrasound contrast agent prepared by this method has the characteristics of uniform size, good developing effect and strong compressive strength. At the same time, its shell material is biodegradable in the body and has no toxic and side effects on human body, so it has broad application prospect.