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以橡单宁为原料 ,通过培养、分离筛选出了一种对单宁具有显著降解作用的菌株 ,经鉴定为内孢霉。通过研究外加碳源、氮源、碳氮比、诱导物及表面活性剂对该菌降解单宁的影响 ,发现这几个因素均明显影响微生物对单宁的降解。该菌在以蔗糖为外加碳源 ,以硝酸钠为氮源 ,碳氮比为 10∶ 1的条件下单宁的降解率最高 ,并发现添加 0 .3× 10 -3 mol/ L的诱导物 2 - 2连氮 - 3-乙苯 -二噻唑 - 6硫酸 (ABTS)、0 .2 g/ L的表面活性剂吐温 - 80 (Tw- 80 )及十二烷基磺酸钠 (SDS)有助于单宁的降解
With rubber tannin as raw material, a strain of tannin with significant degradation was screened through culture and isolation, and was identified as endosporidium. The effects of external carbon source, nitrogen source, C / N ratio, inducer and surfactant on the degradation of tannin were studied. It was found that these factors significantly affected the degradation of tannin by microorganisms. The strain had the highest degradation rate of tannin with sucrose as the carbon source and sodium nitrate as the nitrogen source with the ratio of carbon to nitrogen of 10: 1, and found that when the inducer was added at 0.3 × 10 -3 mol / L, (ABTS), Tween - 80 (Tw - 80) and Sodium Dodecyl Sulfate (SDS) with 0.2 g / L of 2 - Helps to degrade tannins