论文部分内容阅读
目的 通过对Ⅰ型ＪＢＰ（ｃ 型） 和Ⅲ型６７１５（ｇ 型） 变链菌在不同状态葡糖基转移酶（ＧＴＦ） 所合成的水不溶性葡聚糖（ＩＧ） 和水溶性葡聚糖（ＳＧ） 的测定来研究两种变链菌的ＧＴＦ－Ｉ和ＧＴＦ－ Ｓ是否存在差异。方法 制备ＪＢＰ和６７１５ 的含酶上清液（ｃｅｌｌ－ｆｒｅｅ ｅｎｚｙｍｅ） 及含酶提取液（ｃｅｌｌ－ ｂｏｕｎｄ ｅｎｚｙｍｅ），以蔗糖为底物，恒温培养后分别收集ＩＧ 和ＳＧ。采用红外光谱、薄层层析及Ｓｃｏｔｔ 蒽酮定糖法对葡聚糖样品作定性、定量分析。结果 Ⅰ型ＪＢＰ（ｃ）含酶上清液主要合成ＳＧ，含酶提取液主要合成ＩＧ。Ⅲ型６７１５（ｇ）含酶上清液主要合成ＩＧ，含酶提取液合成ＩＧ稍多于ＳＧ，但无统计学显著性差异。对于含酶上清液ＩＧ 的合成，６７１５ 明显多于ＪＢＰ（Ｐ＜ ０ ．０５） ，而ＳＧ 的合成，ＪＢＰ明显多于６７１５（ Ｐ＜ ０．０５） 。含酶提取液ＩＧ的合成，ＪＢＰ明显多于６７１５（ Ｐ＜ ０．０５），而ＳＧ 的合成，６７１５明显多于ＪＢＰ（Ｐ＜ ０．０５）。结论 变链菌Ⅰ型ＪＢＰ的ＩＧ主要由细胞结合型ＧＴＦ 所合成，而变链菌Ⅲ型６７１５ 的ＩＧ主要由细胞游离型ＧＴＦ所合成。这种来自不同型ＧＴＦ 的ＩＧ 是否对变?
OBJECTIVE: To investigate the effects of glucanotransferase (GTF) on type Ⅰ JBP (type C) and type III 6715 (type g) Streptococcus mutans on the expression of water insoluble glucan (IG) and water soluble glucan SG) to investigate whether there is a difference between GTF-I and GTF-S of two S. mutans. Methods Cell-free enzyme and cell-bound enzyme of JBP and 6715 were prepared. The sucrose was used as a substrate and incubated for IG and SG respectively. The dextran samples were qualitatively and quantitatively analyzed by IR, TLC and Scott anthrone. Results Type Ⅰ JBP (c) contained enzyme supernatant mainly synthesizing SG, and enzyme-containing extract mainly synthesized IG. Type III 6715 (g) The enzyme-containing supernatant mainly synthesizes IG, the enzyme-containing extract IG slightly more than the synthesis of SG, but no statistically significant difference. For the synthesis of enzyme-containing supernatant IG, 6715 was significantly more than JBP (P <0.05), while SG synthesis was significantly greater than 6715 (P <0.05). The synthesis of enzyme-containing extract IG, JBP was significantly more than 6715 (P <0.05), while the synthesis of SG, 6715 was significantly more than JBP (P <0.05). Conclusion IG of Streptococcus mutans Ⅰ is mainly synthesized by cell-bound GTF, while IG of Streptococcus mutans Ⅲ 6715 is mainly synthesized by cell-free GTF. Is this kind of IG from different GTFs changing?