目的:考察淫羊藿不同提取物的体外抑菌作用。方法:采用倍比稀释法考察淫羊藿不同提取物对金黄色葡萄球菌、大肠埃希菌、白色念珠菌和枯草芽孢杆菌的抑菌作用,测定抑菌圈大小、最小抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:淫羊藿提取物对金黄色葡萄球菌、大肠埃希菌、白色念珠菌和枯草芽孢氏菌均呈现不同程度的抑菌能力,水提物的抑菌能力强于醇提物(P<0.05或P<0.01);水提物经过不同温度热处理后对各菌株的抑菌能力无显著差异(P>0.05),醇提物经高温处理后抑菌能力呈下降趋势,温度越高抑菌能力越弱(P<0.05)。淫羊藿水提物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌的MIC和MBC相同,分别为7.81,1.95,31.25 mg·ml~(-1),白色念珠菌的MIC和MBC分别为15.63,31.25 mg·ml~(-1);淫羊藿醇提物对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌、白色念珠菌的MIC和MBC相同,分别为15.63,3.91,62.5,62.5 mg·ml~(-1)。结论:淫羊藿提取物具有明显的抑菌效果,其中水提物优于醇提物。淫羊藿不同化学成分的极性会对抑菌效果产生影响。 Objective: To study the antibacterial effect of different extract of Epimedium in vitro. Methods: The antibacterial effect of different extracts of Epimedium on Staphylococcus aureus, Escherichia coli, Candida albicans and Bacillus subtilis was investigated by fold ratio dilution method. The inhibition zone size, minimum inhibitory concentration (MIC) And minimum bactericidal concentration (MBC). Results: Epimedium extract showed different degree of antibacterial activity against Staphylococcus aureus, Escherichia coli, Candida albicans and Bacillus subtilis, and the antibacterial ability of aqueous extract was stronger than that of alcohol extract (P < 0.05 or P <0.01). The antibacterial activity of the water extracts after heat treatment at different temperatures showed no significant difference (P> 0.05). The antibacterial activity of alcohol extracts showed a decreasing trend after high temperature treatment. Weak ability (P <0.05). The MIC and MBC of Epimedium water extract were the same as that of Staphylococcus aureus, Escherichia coli and Bacillus subtilis, they were 7.81, 1.95 and 31.25 mg · ml -1, MIC and MBC of Candida albicans respectively 15.63 and 31.25 mg · ml -1, respectively. The MICs and MBC of the extract of Epimedium were the same as that of Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Candida albicans, which were 15.63, 3.91 and 62.5 , 62.5 mg · ml -1. Conclusion: Epimedium extract has obvious antibacterial effect, of which water extract is better than alcohol extract. Epimedium different chemical composition of the polarity of the antibacterial effect will have an impact.