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目的分析不同压强CO2气腹对结肠癌细胞表面粘附分子表达的影响以及在此基础上对癌细胞粘附能力的影响。方法建立体外气腹模型,选用人结肠癌细胞株SW 1116,分别在6mmHg(1mmHg=0.133 kPa)、9mmHg、12mmHg和15mmHg压强CO2以及常规条件下暴露1h后,流式细胞技术(FACScan)检测粘附分子上皮钙黏着素(E-cadherin)、细胞间粘附分子-1(ICAM-1)、CD44和E-选择素(E-selectin)于处理后0h、12h、24h、48h和72h在结肠癌细胞表面的表达;取各组处理后0 h和72 h细胞进行体外粘附试验。结果经各组压强CO2气腹处理后,SW 1116表面各粘附分子的表达与处理前相比变化有显著性(P<0.05),这些变化在各压强CO2处理后72h之前均恢复至处理前水平,或低于处理前水平(P<0.05)。在处理后0h,随CO2气腹压强增高,E-cadherin、CD44和ICAM-1表达量降低,差异均有显著性(P<0.05),结肠癌细胞的粘附能力也呈下降的趋势(P<0.05),处理后72 h,各压强组之间的细胞粘附差异无显著性。结论CO2气腹对结肠癌细胞表面粘附分子的表达可产生一过性双向影响,随CO2气腹压强的增高,癌细胞粘附分子的表达与粘附能力均受到抑制。
Objective To analyze the effect of different CO2 pneumoperitoneum on the expression of adhesion molecules on colon cancer cell surface and the effect on adhesion of cancer cells. Methods The pneumoperitoneum model was established in vitro. The human colon cancer cell line SW1116 was selected and tested for its ability to detect FACScan by flow cytometry (FACScan) after being exposed to CO2 at 6mmHg (1mmHg = 0.133kPa), 9mmHg, 12mmHg and 15mmHg for 1h, respectively. The expressions of E-cadherin, ICAM-1, CD44 and E-selectin in the colon were detected at 0h, 12h, 24h, 48h and 72h after treatment The expression of cancer cell surface was measured. The cells were in vitro tested at 0 h and 72 h after treatment. Results After CO2 pneumoperitoneum treatment, the expression of each adhesion molecule on the surface of SW 1116 was significantly different from that before treatment (P <0.05), and these changes were restored to before treatment 72 hours before each pressure CO2 treatment Level, or below pre-treatment levels (P <0.05). At 0h after treatment, the expression of E-cadherin, CD44 and ICAM-1 decreased with the increase of CO2 pneumoperitoneum pressure, the difference was significant (P <0.05), and the adhesion ability of colon cancer cells also decreased <0.05). There was no significant difference in cell adhesion between each pressure group at 72 h after treatment. Conclusions CO2 pneumoperitoneum has a transient bidirectional effect on the expression of adhesion molecules on colon cancer cells. With the increase of CO2 pneumoperitoneum pressure, the adhesion molecules expression and adhesion ability of cancer cells are inhibited.