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目的探讨蒿甲醚对人结直肠癌细胞株HCT116及HT29的细胞毒性和放射增敏作用。方法采用MTT法检测蒿甲醚对人结直肠癌细胞株HCT116及HT29的细胞毒性。克隆形成实验检测蒿甲醚对人结直肠癌细胞株HCT116及HT29的放射增敏作用,多靶单击模型拟合HCT116及HT29细胞的剂量存活曲线,计算蒿甲醚对HCT116及HT29细胞的放射增敏比,评价其放射增敏效果。结果蒿甲醚对HCT116及HT29细胞作用24 h、48 h、72 h的IC50分别为:HCT116:300μg/ml、270μg/ml、170μg/ml;HT29:280μg/ml、250μg/ml、200μg/ml。蒿甲醚对HCT116及HT29细胞在平均致死剂量的放射增敏比(SERD0)分别为1.31和1.25。结论蒿甲醚对人结直肠癌细胞株HCT116及HT29的细胞毒性作用呈剂量和时间依赖性,并可对人结直肠癌细胞系HCT116及HT29有放射增敏作用。
Objective To investigate the cytotoxicity and radiosensitization of artemether on human colorectal cancer cell lines HCT116 and HT29. Methods The cytotoxicity of artemether against human colorectal cancer cell lines HCT116 and HT29 was detected by MTT assay. The colony formation assay was used to detect the radiosensitization effect of artemether on human colorectal cancer cell lines HCT116 and HT29. The multi-target click model was fitted to the dose-survival curve of HCT116 and HT29 cells to calculate the radiation of HCT116 and HT29 cells Sensitization ratio, evaluate the radiosensitization effect. Results IC50 of HCT116: 300μg / ml, 270μg / ml and 170μg / ml for HT29: 280μg / ml, 250μg / ml, 200μg / ml for 24 h, . The mean lethal dose-response (SERD0) of artemether to HCT116 and HT29 cells was 1.31 and 1.25, respectively. Conclusions Artemether has dose-and time-dependent cytotoxicity on human colorectal cancer cell lines HCT116 and HT29, and radiosensitises human colorectal cancer cell lines HCT116 and HT29.