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目的建立HPLC法同时测定复方儿茶止泻膜剂中儿茶素、表儿茶素和没食子酸3个成分的方法。方法色谱柱为Hypersil ODS(250 mm×4.6 mm,5μm),流动相为乙腈-50 mmol/L磷酸二氢钾(8∶92),体积流量为1.0mL/min,检测波长280 nm,柱温为30℃。结果3个成分分离良好,儿茶素、表儿茶素和没食子酸的平均回收率分别为97.5%、98.4%、102.3%。结论本法方便、准确、重现性好,可以作为复方儿茶止泻膜剂质量控制的方法。
OBJECTIVE To establish a HPLC method for the simultaneous determination of catechin, epicatechin and gallic acid in compound catechin antidiarrheal film. Methods The column was Hypersil ODS (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-50 mmol / L potassium dihydrogen phosphate (8:92), the volume flow rate was 1.0 mL / min and the detection wavelength was 280 nm. Is 30 ° C. Results The three components were well separated. The average recoveries of catechin, epicatechin and gallic acid were 97.5%, 98.4% and 102.3%, respectively. Conclusion This method is convenient, accurate and reproducible. It can be used as a quality control method for compound catechin antidiarrheal film.