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以沙坡头生物结皮(BSC)为研究对象,采用Biolog鉴定系统,通过碳源利用分析、功能多样性分析和聚类分析,对BSC微生物的多样性进行了研究。结果表明:BSC微生物群落代谢的平均颜色变化率(AWCD)在培养初期(0～24h)值很小处于适应期,中后期(24～168 h)AWCD值逐渐增强处于指数期,可能由于监测时间不是足够长,所以稳定期和衰亡期表现得不明显;BSC微生物能够利用羧酸类、聚合物类、碳水化合物类、酚类化合物类、氨基酸类和胺类6类碳源,其中对羧酸类碳源的利用率最高,对胺类碳源的利用率最低;BSC中微生物群落多样性指数为3.99,微生物群落均匀度指数为1.44;指纹聚类分析表明,当聚合水平>8.6时,BSC微生物可分为6类。研究表明,Biolog技术能够简便、快速、有效地反映BSC中的微生物活性及其变化情况。
Using BSC as the research object, Biolog identification system was used to study the diversity of BSC microorganisms through carbon source utilization analysis, functional diversity analysis and cluster analysis. The results showed that the mean color change rate (AWCD) of metabolic rate of BSC microbial community was very low at the initial stage of culture (0-24 h), and gradually increased at the mid-late stage (24-168 h), which was probably due to the monitoring time BSC microorganisms can utilize carboxylic acids, polymers, carbohydrates, phenolic compounds, amino acids and amines Class 6 carbon sources, which carboxylic acid The utilization rate of carbon source was the highest, and the utilization rate of amine carbon was the lowest. The diversity index of microbial community in BSC was 3.99 and the evenness index of microbial community was 1.44. Fingerprint cluster analysis showed that when the polymerization level was> 8.6, BSC Microorganisms can be divided into 6 categories. Research shows that Biolog technology can reflect the microbial activity and its change in BSC easily, quickly and effectively.