论文部分内容阅读
构建了含有Bt杀虫基因cry1Ah和耐草甘膦基因2mG2-epsps的双价植物表达载体pMAGUHM,通过基因枪轰击Q31×Z31杂交组合的胚性愈伤组织,以2mG2-epsps为筛选标记,通过1mmol/L草甘膦异丙胺盐筛选,获得T0代转化植株80株,其中PCR检测有36株为阳性植株,阳性率达45%。对得到的阳性植株的T1、T2代进行了跟踪检测,证明外源基因已整合到玉米基因组中并正确表达。草甘膦及玉米螟抗性检测结果表明,有5个转基因品系表现出具有耐草甘膦和抗虫的双重特性。
A bivalent plant expression vector pMAGUHM containing Bt insecticidal gene cry1Ah and glyphosate tolerant gene 2mG2-epsps was constructed. The embryogenic callus of Q31 × Z31 cross was bombarded with the gene gun, and the marker was selected by 2mG2-epsps 1 mmol / L glyphosate isopropylamine salt screening, access to T0 generation of 80 transformed plants, of which 36 were PCR-positive plants, the positive rate of 45%. The T1 and T2 generation of the positive plants were followed up, which proved that the foreign gene has been integrated into the maize genome and correctly expressed. The test results of glyphosate resistance and Ostrinia furnacalis showed that five transgenic lines showed the dual characteristics of glyphosate resistance and insect resistance.