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目的建立银翘袋泡茶的质量控制方法。方法采用显微鉴别法鉴别方中的金银花;采用薄层色谱法对方中的金银花、广藿香进行定性鉴别;采用高效液相色谱法测定方中连翘苷的含量。结果金银花的花粉粒显微特征清晰,专属性好;金银花、广藿香的薄层色谱斑点清晰、分离度好,且各阴性对照无干扰;连翘苷在进样量为0.51~3.06μg范围内线性关系良好(r=0.999 9),平均加样回收率为98.85%,RSD为1.51%(n=6)。结论该方法专属性强、灵敏度高、重复性好,可用于银翘袋泡茶的质量控制。
Objective To establish the quality control method of Yin-berg tea. Methods The honeysuckle was identified by microscopic identification method. The honeysuckle and patchouli were identified by TLC, and the content of forsythin by HPLC was determined by HPLC. Results The honeysuckle pollen grains had distinct microscopic features and good specificity. The TLC spots of honeysuckle and patchouli were clear, with good resolution and no interference with each negative control. The forsythin in the range of 0.51-3.06 μg The linearity was good (r = 0.999 9). The average recovery was 98.85% and the RSD was 1.51% (n = 6). Conclusion The method is highly specific, sensitive and reproducible. It can be used for quality control of Yincao teabag.