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在制作各种动物脑干或小动物全脑火棉胶切片时,按照传统的方法,一般都要浸胶2—3个月,甚至一年以上。这样旷时日久的方法,对于当前科研工作很不适应。今年我们在对研究生、进修生进行基本神经解剖技术方法培训时,采用了空气压缩浸胶的方法制作了一批新生儿及猴脑干,猫、兔、大鼠全脑的火棉胶(国产硝化纤维)连续切片。实践证明这种方法是可行的,可以大大缩短制作时间。现将此方法的步骤简述于下:
In the production of various animal brainstem or small animal whole brain fire cotton gel slices, according to the traditional method, generally 2-3 months dipped, or even more than one year. Such a long way to go is not suitable for the current scientific research work. This year, we trained graduate students and advanced students in the method of basic neuroanatomy and adopted a method of air compression dipping to make a batch of collodion of domesticated brain, cat, rabbit and rat Nitrocellulose) serial sections. Practice has proved that this method is feasible, can greatly shorten the production time. Now the method steps outlined below: