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为优化金线莲黄酮提取工艺,根据单因素试验结果与响应面分析法中的Box-Behnken中心组合设计原则选取试验因素与水平,从中选取对叶绿素提取结果有影响的提取时间、提取温度、乙醇体积分数、液料比4个因素进行优化;并利用Design Expert 7.1.6分析软件对试验数据进行分析。结果表明,影响金线莲总黄酮含量的因素大小顺序为:提取温度、乙醇体积分数、提取时间、液料比,金线莲黄酮提取的最佳工艺参数为提取温度62.25℃,乙醇浓度为80.68%,提取时间89.8 min,液料比为79.38∶1;在此条件下,金线莲黄酮含量为2.243 7 mg/g,与预测值2.338 6 mg/g接近。采用响应面分析法得到的金线莲黄酮提取优化工艺准确可靠、可行,可用于金线莲总黄酮的提取,为金线莲资源的进一步开发利用提供了实验依据。
In order to optimize the extraction process of flavonoids from Lonicera chinensis, the factors and levels of Box-Behnken center design were selected according to the combination of single factor test and response surface analysis. The extraction time, extraction temperature, ethanol Volume fraction, liquid to liquid ratio of four factors to optimize; and Design Expert 7.1.6 analysis software to analyze the experimental data. The results showed that the order of factors influencing the content of total flavonoids in A. japonicus was extraction temperature, ethanol volume fraction, extraction time and liquid to material ratio. The optimum extraction parameters were as follows: extraction temperature 62.25 ℃, ethanol concentration 80.68 %, The extraction time was 89.8 min, the ratio of liquid to material was 79.38:1. Under this condition, the content of flavonoids in A. sinense was 2.243 7 mg / g, close to the predicted value of 2.338 6 mg / g. The optimal extraction process of flavonoids from A. officinarum by response surface methodology was accurate, reliable and feasible, which could be used to extract the total flavonoids of A. officinarum and provided an experimental basis for the further development and utilization of the A. wilfordii resources.