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为研究1,3-丁二醇对大鼠的亚慢性毒性作用及其可逆性,将200只健康SPF级SD大鼠随机分为4组,分别为对照(灭菌水)组和低(500 mg/kg)、中(1 000 mg/kg)剂量1,3-丁二醇染毒组及高剂量(2 000 mg/kg)1,3-丁二醇染毒解剖组和恢复(染毒结束后继续观察28 d)组,每组20只,雌雄各半。观察动物的一般情况,测定体重和食物消耗情况及主要脏器重量及其脏器系数,测定血液学和血清生化学指标,进行大体解剖并对主要脏器进行组织病理学检查。结果显示,经口和经皮染毒后,中、高剂量组大鼠出现短暂嗜睡,未见其他明显异常;各剂量1,3-丁二醇染毒组大鼠的体重、食物利用率及各主要脏器系数与对照组比较,差异均无统计学意义(P>0.05);与对照组比较,仅高剂量1,3-丁二醇亚慢性经皮染毒解剖组雄性大鼠外周血白细胞总数和丙氨酸氨基转移酶水平均较高,差异均有统计学意义(P>0.05);停止染毒28 d后恢复正常;未见与受试物相关的病理改变。提示在本试验条件下,1,3-丁二醇亚慢性经口、经皮毒性试验无有害作用水平(NOAEL)分别为2 000、1 000 mg/kg。
To investigate the subchronic toxicity and reversibility of 1,3-butanediol in rats, 200 healthy SPF SD rats were randomly divided into 4 groups: control (sterile water) group and low (500) mg / kg), 1,3-butanediol (1 000 mg / kg) and 1,3-butanediol (2 000 mg / After the end of observation continue 28 d) group, each group of 20, male and female. Observed the general situation of animals, body weight and food consumption and the weight of the major organs and their organ coefficients, hematology and serum biochemical markers were measured, gross anatomy and histopathological examination of major organs. The results showed that after oral and transdermal administration, the rats in middle and high dose group had transient drowsiness and no other obvious abnormalities. The body weight and food utilization rate of 1,3-butanediol-treated rats Compared with the control group, only the high-dose 1,3-butanediol subcutaneous transdermal drug exposure group of male rats peripheral blood The total number of leukocytes and alanine aminotransferase levels were higher, the difference was statistically significant (P> 0.05); stopped 28 days after exposure to normal; no pathological changes associated with the test substance. Tip Under the test conditions, 1,3-butanediol sub-chronic oral, dermal toxicity test NOAEL were 2000, 000 mg / kg.