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目的建立骨复康胶囊中龙血素A的含量测定方法。方法采用HPLC法测定骨复康胶囊中龙血素A的含量。Kromasil C18(4.6 mm×250 mm,5μm)色谱柱;甲醇-1%冰醋酸溶液(55:45)为流动相;检测波长为280 nm;流速为1.0 l/min;柱温:35℃。结果龙血素A在0.476～4.284μg范围内呈现良好的线性关系;回收率为99.51%,RSD为0.78%(N=6)。结论该方法简便、准确,重现性好,可用于骨复康胶囊的质量控制。
OBJECTIVE To establish a method for the determination of Loureirin A in Gufukang capsule. Methods The content of foraronin A in Gufukang capsule was determined by HPLC. Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol-1% acetic acid solution (55:45), the detection wavelength was 280 nm, the flow rate was 1.0 l / min and the column temperature was 35 ℃. Results Loureirin A showed a good linear relationship in the range of 0.476 ~ 4.284 μg. The recovery rate was 99.51% and the RSD was 0.78% (N = 6). Conclusion The method is simple, accurate and reproducible and can be used for the quality control of Gufukang capsule.