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目的 :探讨不同类型高脂血症患者血清高密度脂蛋白 (HDL)亚类组成和相对百分含量的变化及其之间的差异。方法 :采用双向电泳 免疫印迹检测法分析了 39例高胆固醇 (TC)血症患者、97例高甘油三酯 (TG)血症患者及 32例混合性高脂血症 (MHL)患者血清HDL亚类组成及相对百分含量。结果 :高TC、高TG及MHL患者血清中小颗粒的前 β1 HDL、前 β2 HDL、及HDL3b 含量显著增加 (P<0 .0 5 ～ <0 .0 1) ,而大颗粒的HDL2a及HDL2b含量显著减少 (P <0 .0 5～<0 .0 1)。高TC患者血清中HDL2b含量显著高于高TG及MHL患者 (P <0 .0 1及P<0 .0 5 ) ,而前 β1 HDL含量低于或显著低于高TG及MHL患者 (P<0 .0 1) ;高TG患者血清中HDL3a含量显著高于高TC及MHL患者 (P<0 .0 1) ,而HDL2b含量均较其他二者为低。结论 :高TC、高TG及MHL患者血清HDL颗粒直径均呈变小趋势 ,但其HDL亚类组成的具体变化特征却有一定差异。
Objective: To investigate the changes of the composition and relative percentage of serum high density lipoprotein (HDL) in different types of patients with hyperlipidemia and their differences. Methods: Two-dimensional electrophoresis and Western blotting were used to analyze the serum levels of HDL in 39 patients with hypercholesteremia (TC), 97 patients with hypertriglyceridemia (TG) and 32 patients with mixed hyperlipidemia (MHL) Class composition and relative percentage. Results: The levels of pre-β1 HDL, pre-β2 HDL and HDL3b in the serum of high TC, high TG and MHL patients increased significantly (P <0.05 ~ <0.01), while the contents of HDL2a and HDL2b Significantly decreased (P <0.05 ~ <0.01). The serum HDL2b levels in patients with high TC were significantly higher than those in patients with high TG and MHL (P <0.01 and P <0.05), while the levels of pre-β1 HDL were lower or significantly lower than those in patients with high TG and MHL (P < 0. 01). The serum HDL3a levels in patients with high TG were significantly higher than those in patients with high TC and MHL (P <0.01), while HDL2b levels were lower than those in the other two groups. CONCLUSIONS: The HDL particle diameters of high TC, high TG and MHL patients tend to decrease, but the specific characteristics of HDL subclass composition have some differences.