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本实验用荧光测定法研究金葡球菌对环丙沙星、托氟沙星的摄入及能量抑制剂对细菌摄入药物的影响。结果显示:耐药菌对环丙沙星摄入量低于敏感菌,两者对托氟沙星的摄入量没有显著性差异;加入能量抑制剂后,所有细菌对环丙沙星的摄入量均有不同程度增加,除2株敏感菌外,细菌对托氟沙星的摄入没有明显增加。诱导菌对两种药物的摄入量均低于母株:能量抑制剂对细菌摄入环丙沙星、托氟沙星的影响明显不同。表明摄入减少是细菌对两类氟喹诺酮耐药的机制之一,而泵出增加导致的摄入减少仅适合亲水性氟喹诺酮,对疏水性氟喹诺酮目前还难以定论。
In this experiment, the influence of Staphylococcus aureus on the intake of ciprofloxacin and tolloxacin and the energy inhibitor on bacterial ingestion were studied by fluorescence assay. The results showed that the drug-resistant bacteria had lower intake of ciprofloxacin than the sensitive ones, and there was no significant difference in the intake of tetracycline between the two strains. After the energy inhibitor was added, all the bacteria took ciprofloxacin In addition to the two strains of bacteria, the bacteria did not significantly increase the intake of tolloxacin. Induced bacteria in both drugs intake were lower than the parent strain: energy inhibitors on the bacterial intake of ciprofloxacin, toloxacin significantly different. Decrease in intake is one of the mechanisms by which bacteria respond to two types of fluoroquinolones, and the decrease in ingestion due to increased pumping is only suitable for hydrophilic fluoroquinolones, and hydrophobic fluoroquinolones are currently difficult to determine.