【摘 要】
：
运用20个10碱基随机引物,对中国龙脑香科(Dipterocarpaceae)特有的珍稀濒危植物版纳青梅(Vatica guangxiensis X. L. Mo)进行了RAPD多态性分析.3个自然居群和1 个迁地保护居
【机 构】
：
中国科学院西双版纳热带植物园,中国科学院北京植物研究所系统与进化植物学开放研究实验室,Xishuangbanna Tropical Botanical Garden,Kunming Institute
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运用20个10碱基随机引物,对中国龙脑香科(Dipterocarpaceae)特有的珍稀濒危植物版纳青梅(Vatica guangxiensis X. L. Mo)进行了RAPD多态性分析.3个自然居群和1 个迁地保护居群(分布于云南和广西)共扩增出231个位点,多态位点所占比例(PPB)为53.68%;观察等位基因数na=1.536*!8,有效等位基因数ne=1.287*!8,Nei基因多样性指数h为0.168*!6,居群内的遗传多样性水平较低.基于AMOVA和POPGENE的结果均表明居群内的遗传变异大于居群间的遗传变异.居群内的遗传变异为55.09%,居群间的变异为44.91% (AMOVA);基因分化系数Gst为0.374*!6 (POPGENE),表明居群间存在高水平的遗传分化.研究结果对该濒危植物的保护有重要意义.考虑到低水平的遗传多样性和高水平的居群分化,通过居群间种子和幼苗的交换来促进基因流是可行的保护方案.迁地保护居群(ML)不具最高的遗传多样性,表明为了保护此濒危物种的全部遗传变异,需要进一步采集更多个体补充到迁地保护居群中.
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