论文部分内容阅读
目的通过流式细胞仪(FCM)技术测定胸水脱落细胞细胞核的脱氧核糖核酸(DNA)含量,并将此方法与传统的病理学方法,肿瘤标记物癌胚抗原(CEA)的检测相比较,以鉴别胸水的良恶、性。方法病例选自2003-03~2004-03苏州大学附属第二医院呼吸内科的住院患者,留取新鲜的胸腔积液标本,以肝素抗凝,荧光染料染色后,用流式细胞仪检测细胞核DNA倍体,分析细胞的增殖能力。结果根据临床检查将胸水分为良性和恶性两组,在恶性胸腔积液组中,87·5%的标本可以检测到DNA异倍体,在良性胸腔积液中,90%的标本表现为两倍体。结论流式细胞仪DNA倍体测定使得病理诊断有了可依据的客观指标,是病理学检查的有益补充,与CEA联合测定可以提高恶性胸腔积液的检出率。
OBJECTIVE: To determine the DNA content of exfoliated cells by flow cytometry (FCM) and compare the results with traditional pathological methods and tumor markers CEA Identification of benign and malignant pleural effusion, sex. Methods The patients were selected from inpatients of Department of Respiratory Medicine of the Second Affiliated Hospital of Soochow University from March 2003 to March 2004. Fresh pleural effusion specimens were obtained. After heparin anticoagulation and fluorescent dye staining, the nuclear DNA was detected by flow cytometry Ploidy, analysis of cell proliferation. Results According to clinical examination, pleural effusion was divided into benign and malignant groups. In malignant pleural effusion group, 87.5% of specimens could detect DNA aneuploidy. In benign pleural effusion, 90% of specimens showed two Ploidy. Conclusion Flow cytometry DNA ploidy determination makes the pathological diagnosis can be based on objective indicators, is a useful complement to pathological examination, combined with CEA can increase the detection rate of malignant pleural effusion.