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运用流式细胞仪和膜片钳技术研究La3+,Gd3+,Yb3+三种稀土离子诱导大鼠背根神经元DRG凋亡以及膜上钾离子通道的影响。结果表明:10,100,1000μmol.L-1LaCl3和GdCl3处理DRG神经元96 h,细胞不出现凋亡;YbCl3处理96 h,细胞的凋亡率明显递增。胞外La3+,Gd3+,Yb3+抑制瞬间外向钾电流IA,使IA的激活和失活过程都显著右移,抑制和右移的程度La3+最弱,Gd3+次之,Yb3+最强;胞内的La3+,Gd3+,Yb3+抑制延迟整流钾电流IK,抑制的程度也呈增长趋势。稀土离子在胞内外的结合位点不同;Yb3+较La3+和Gd3+的神经毒性强。
The effects of La3 +, Gd3 + and Yb3 + on the DRG apoptosis and the K (superscript +) channel in the DRG neurons were investigated by flow cytometry and patch-clamp technique. The results showed that 10, 100, 1000μmol.L-1LaCl3 and GdCl3 treatment of DRG neurons 96 h, the cells did not show apoptosis; YbCl3 treatment 96 h, the cell apoptosis rate was significantly increased. Extracellular La3 +, Gd3 + and Yb3 + inhibited transient outward potassium current IA, which significantly shifted IA activation and inactivation to the right. Inhibition and right shift were the weakest in La3 +, Gd3 + followed by Yb3 + Gd3 +, Yb3 + inhibit the delayed rectifier potassium current IK, the degree of inhibition also showed an increasing trend. Rare earth ion binding sites in different cells; Yb3 + than La3 + and Gd3 + neurotoxicity.