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自1964年 Beutner 等观察到寻常性天疱疮和落叶性天疱疮患者血清中存在细胞间质抗体以来,对这些抗体能否固定补体一直存在分歧,不能确定补体系统在本病中的作用。作者用间接免疫荧光法(IIF)和补体免疫荧光法(CIF)检测了20例巴西落叶性天疱疮患者血清抗细胞间质(IC)抗体和补体结合抗体。考虑到 IgG 4个亚类的不同的补体结合力(IgG4无补体结合力),故对 IC 抗体应用单克隆抗体作了亚类分析,以研究 IC 抗体的 IgG亚类与固定补体能力的关系。结果,大多数患者(19/20)检出 IC 抗体,但其中 CIF 阳性仅4例。IgG 亚类分析表明,CIF 阴性的 IC 抗体仅1例属IgG4,其余均至少属 IgG 1～IgG 3中的一个亚类。CIF 阳性血清中有1例未检出 IC 抗体,其余
Since Beutner et al. Observed in 1964 the presence of interstitial antibodies in the sera of patients with pemphigus vulgaris and pemphigus pemphigus, it was not clear whether these antibodies could be involved in the fixation of complement and could not determine the role of the complement system in this disease. The authors used indirect immunofluorescence (IIF) and complement immunofluorescence (CIF) to detect serum anti-ICAM and complement-binding antibodies in 20 patients with Brazilian folic pemphigus. Taking into account the different complement binding forces (IgG4 non-complement binding) of the four subclasses of IgG, a subclass analysis was performed using monoclonal antibodies to IC antibodies to investigate the relationship of IgG subclasses of IC antibodies to immobilized complement. As a result, IC antibodies were detected in most patients (19/20), but only 4 were positive for CIF. IgG subclass analysis showed that only one CIF-negative IC antibody was IgG4, and the rest were at least one subclass of IgG1-IgG3. One of the CIF-positive sera did not detect IC antibody, and the rest