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建立一种快速、有效的高效液相色谱法测定运动食品中丙二醛的方法。样品经三氯乙酸混合提取液提取后,与TBA在90℃温度下衍生20 min。以乙酸铵(20 mmol/L)-乙腈(30∶70,V/V)作为流动相,用荧光检测器检测,外标法峰面积定量。结果表明,丙二醛标液在0.1~4.0μg/mL浓度范围内线性良好,相关系数r~2为0.999 2,检出限为0.02 mg/kg,定量限为0.06 mg/kg。加标回收率的添加浓度为1.0,5.0和10.0 mg/kg,加标回收率达到90.4%~93.1%(n=5)。通过模拟样品酸败的过程,与过氧化值相比,丙二醛值更能反应样品的氧化程度。
To establish a rapid and effective method for the determination of malondialdehyde in sports food by high performance liquid chromatography. The sample was extracted with trichloroacetic acid and extracted with TBA for 20 min at 90 ℃. Ammonium acetate (20 mmol / L) -acetonitrile (30:70, V / V) was used as the mobile phase. The fluorescence detector was used to detect the peak area. The results showed that the calibration curve of malondialdehyde was linear in the concentration range of 0.1-4.0μg / mL with the correlation coefficient r ~ 2 of 0.999 2, the detection limit of 0.02 mg / kg and the limit of quantification of 0.06 mg / kg. The spiked recoveries were 1.0, 5.0 and 10.0 mg / kg, and the spiked recoveries ranged from 90.4% to 93.1% (n = 5). By simulating the process of rancidity, the value of malondialdehyde reacts more with the degree of oxidation than the peroxide value.