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为明确河北省推广种植植转Bt基因抗虫棉(简称Bt棉)后,棉铃虫Helicoverpa armigera(Hübner)对常用杀虫剂的抗药性水平及其生化机理,2011-2012年采用点滴法对保定南郊、沧州南皮、邢台巨鹿3个地区的田间种群以及敏感种群进行了室内毒力测定,并采用生化分析法对4个种群相关的羧酸酯酶(carboxylesterase,CarE)、谷胱甘肽S-转移酶(glutathione S-transferases,GSTs)和乙酰胆碱酯酶(acetylcholinesterase,AChE)的活性进行了研究。结果表明:3个田间种群对高效氯氰菊酯和氰戊菊酯处于中至高抗水平,抗性倍数为20.02～73.70倍;对灭多威处于低至中抗水平,抗性倍数为6.27～11.84倍;对高效氯氟氰菊酯(抗性倍数:1.07～4.20倍),辛硫磷、毒死蜱和马拉硫磷(抗性倍数:1.00～2.69倍),以及氯虫苯甲酰胺(抗性倍数:2.00～3.67倍)均处于敏感水平。3个田间种群的CarE,GSTs和AChE活性分别是敏感种群的1.06～1.23,1.20～1.63和1.15～1.23倍,这可能与其对高效氯氰菊酯、氰戊菊酯和灭多威产生的抗性有关。
In order to clarify the resistance level and biochemical mechanism of Helicoverpa armigera (Hübner) against common insecticides after planting and transplanting the transgenic Bt cotton (referred to as Bt cotton) in Hebei Province for 2011-2012, The indoor population and the susceptible population of three populations in southern suburbs, Cangzhou Nanpi and Xingtai Julu were tested for their indoor toxicity. Four populations of carboxylesterase (CarE), glutathione S-transferases (glutathione S-transferases, GSTs) and acetylcholinesterase (AChE) activity were studied. The results showed that the resistance of beta-cypermethrin and fenvalerate to beta-cypermethrin and fenvalerate in the three field populations was 20.02-73.70 times higher than that of methyserothrin and 6.27-11.84 times as high as that of methylobacterium. The effects of lambda-cyhalothrin (resistance fold: 1.07-4.20), phoxim, chlorpyrifos and malathion (resistance fold: 1.00-2.69) and chlorantraniliprole 2.00 ~ 3.67 times) are at a sensitive level. CarE, GSTs and AChE activities in three field populations were 1.06-1.23, 1.20-1.63 and 1.15-1.23 times higher than the susceptible population respectively, which may be related to their resistance to beta-cypermethrin, fenvalerate and methomyl.