在球孢白僵菌不同浓度下,利用饲喂法和浸渍法测试球孢白僵菌对亚洲玉米螟3龄幼虫的致病力,建立球孢白僵菌对亚洲玉米螟毒力生测标准。结果表明,处理组中白僵菌浓度为105、106、107、108 CFU/mL时,采用饲喂法和浸渍法对3龄幼虫的校正死亡率、致死中时间(LT50)、致死中浓度(LC50)均没有显著性差异;饲喂法3龄幼虫的死亡率显著低于浸渍法。因此,球孢白僵菌对亚洲玉米螟毒力生测的常用方法可用饲喂法替代浸渍法,饲喂法试验过程简单,避免反复移动试虫引起的误差,毒力测试结果更可靠。 At different concentrations of Beauveria bassiana, the pathogenicity of Beauveria bassiana to the 3rd instar larvae of the Asian corn borer, Beauveria bassiana, was tested by feeding method and immersion method. The bioassay of Beauveria bassiana to Asiatic corn borer . The results showed that when the concentration of Beauveria bassiana was 105, 106, 107 and 108 CFU / mL, the corrected mortality, the lethal time (LT50) and the lethal concentration LC50) were no significant difference; feeding third instar larvae mortality was significantly lower than the impregnation method. Therefore, Beauveria bassiana on the Asian corn borer virulence commonly used method can be fed instead of immersion method, the feeding method of the test process is simple, to avoid repeated errors caused by test insects, toxicity test results more reliable.