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目的观察黄芪对腹主动脉缩窄慢性压力超负荷所致心力衰竭大鼠心功能的保护作用及其机制。方法雄性SD大鼠80只,随机选取20只大鼠为假手术组,其余60只制作腹主动脉缩窄模型(CAA),随机分为模型组、黄芪组和阳性对照卡托普利组,每组各20只;各组大鼠在术后24 h开始给药,黄芪组每天腹腔注射黄芪注射液2 mL(2 g/kg),假手术组、模型组和卡托普利组每天腹腔内注射2 mL生理盐水,卡托普利组将卡托普利溶入饮水中(100 mg/kg),连续8周;存活大鼠采用血流动力学测定心功能,计算心指数和左心室指数,免疫组织化学法检测大鼠心肌组织中caspase-3表达。结果与模型组[(2.35±0.298)mg/g]比较,黄芪组和卡托普利组大鼠左心室指数[(1.94±0.186)、(1.98±0.256)mg/g]均明显降低(P<0.01);与模型组比较,左心室收缩压(LVSP)、左心室压力上升/下降最大速率(±dp/dtmax)均升高(P<0.05),而左心室舒张末压(LVEDP)降低(P<0.05);与模型组比较,黄芪组和卡托普利组凋亡蛋白caspase-3表达灰度值[(162.3±10.1)、(173.9±11.6)]均降低(P<0.05)。结论黄芪可能通过减少心肌细胞凋亡,进而改善心力衰竭大鼠的心功能及心肌肥大。
Objective To observe the protective effect and its mechanism of Astragalus membranaceus on cardiac function in heart failure rats induced by chronic constriction of abdominal aorta. Methods Eighty male Sprague-Dawley rats were randomly selected. Twenty rats were randomly selected as the sham-operated group and the remaining 60 were given CAA. The rats were randomly divided into model group, Astragalus group and positive control captopril group. 20 rats in each group. Rats in each group began to be administered 24 h after operation. The Astragalus group was given intraperitoneal injection of Astragalus injection 2 mL (2 g / kg) every day. The sham operation group, model group and captopril group Captopril group dissolved captopril into drinking water (100 mg / kg) for 8 weeks, and the survival rats were used to measure the cardiac function by hemodynamics, and the cardiac index and left ventricular The expression of caspase-3 in myocardium was detected by immunohistochemistry. Results Compared with the model group [(2.35 ± 0.298) mg / g], the left ventricular index (1.94 ± 0.186, 1.98 ± 0.256 mg / g) in the Astragalus membranaceus group and Captopril group were significantly decreased <0.01). Compared with the model group, LVSP and ± dp / dtmax increased (P <0.05), and left ventricular end diastolic pressure (LVEDP) decreased (P <0.05). Compared with the model group, the gray value of caspase-3 expression in the Astragalus membranaceus group and Captopril group was significantly lower than that in the model group [(162.3 ± 10.1) and (173.9 ± 11.6)], respectively. Conclusion Astragalus may improve cardiac function and myocardial hypertrophy in heart failure rats by reducing the apoptosis of cardiomyocytes.