【摘 要】
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The xenobiotic transcription factor cap \'n\'collar isoform C (CncC) is con-sidered the central regulator of antioxidant and detoxification genes.Previous research indicated that CncC regulates three-phase enzymes responsible for insecticide resistanc
【机 构】
：
Hunan Provincial Engineering and Technology Research Center for Bio-pesticide and Formulation Proces
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The xenobiotic transcription factor cap \'n\'collar isoform C (CncC) is con-sidered the central regulator of antioxidant and detoxification genes.Previous research indicated that CncC regulates three-phase enzymes responsible for insecticide resistance.In this study,the SlituCncC gene from Spodoptera litura was obtained and characterized.Quantitative polymerase chain reaction (qPCR) analysis showed that SlituCncC was ex-pressed in all developmental stages and tissues,but was highly expressed in 3rd-and 4th-instar larvae,and in the Malpighian tubule,fat body,and midgut.In addition,SlituCncC was up-regulated and more highly induced with indoxacarb treatment in the indoxacarb-resistant strains compared with the susceptible strain.RNA interference-mediated gene silencing of SlituCncC significantly increased mortality of S.litura when exposed to in-doxacarb.Furthermore,comparative transcriptome analysis showed that 842 genes were down-regulated and 127 genes were up-regulated in SlituCncC knockdown S.litura.Fur-ther analysis indicated that 18 three-phase enzymes were identified in the down-regulated genes,of which seven were associated with indoxacarb resistance in S.litura.qPCR anal-ysis confirmed that expression of six of these seven genes was consistent with RNA se-quencing data.All six detoxification genes were induced by indoxacarb,and the expres-sion patterns were similar to that of SlituCncC.Finally,the CncC-Maf binding site was predicted in all six gene promoters.This study indicates that the transcription factor Sli-tuCncC may regulate multiple detoxification genes that mediate indoxacarb resistance in S.litura.
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